Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity

Kazuki Okamoto, Tomoe Ishikawa, Reimi Abe, Daisuke Ishikawa, Chiaki Kobayashi, Mika Mizunuma, Hiroaki Norimoto, Norio Matsuki, Yuji Ikegaya

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Spontaneous neuronal activity is present in virtually all brain regions, but neither its function nor spatiotemporal patterns are fully understood. Ex vivo organotypic slice cultures may offer an opportunity to investigate some aspects of spontaneous activity, because they self-restore their networks that collapsed during slicing procedures. In hippocampal networks, we compared the levels and patterns of in vivo spontaneous activity to those in acute and cultured slices. We found that the firing rates and excitatory synaptic activity in the in vivo hippocampus are more similar to those in slice cultures compared to acute slices. The soft confidence-weighted algorithm, a machine learning technique without human bias, also revealed that hippocampal slice cultures resemble the in vivo hippocampus in terms of the overall tendency of the parameters of spontaneous activity.

Original languageEnglish
Pages (from-to)421-431
Number of pages11
JournalJournal of Physiological Sciences
Volume64
Issue number6
DOIs
Publication statusPublished - 2014 Sep 11
Externally publishedYes

Keywords

  • Machine learning algorism
  • Slice culture
  • Soft confidence-weighted learning
  • Spontaneous activity

ASJC Scopus subject areas

  • Physiology

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  • Cite this

    Okamoto, K., Ishikawa, T., Abe, R., Ishikawa, D., Kobayashi, C., Mizunuma, M., Norimoto, H., Matsuki, N., & Ikegaya, Y. (2014). Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity. Journal of Physiological Sciences, 64(6), 421-431. https://doi.org/10.1007/s12576-014-0337-4