Ex vivo pilot study using computed analysis of endo-cytoscopic images to differentiate normal and malignant squamous cell epithelia in the oesophagus

S. Kodashima, M. Fujishiro, K. Takubo, M. Kammori, S. Nomura, N. Kakushima, Y. Muraki, O. Goto, S. Ono, M. Kaminishi, M. Omata

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Abstract

Background and study aims: An endo-cytoscopy system allows acquisition of optical biopsies that are quite similar to conventional histology. To simplify discrimination between normal and malignant tissue in the oesophagus using endo-cytoscopy system, we analysed the nuclear (dark staining) area in the obtained images with the goal of an accurate, automatic diagnosis. Patients and methods: Ex vivo endo-cytoscopic observation was performed using endoscopically or surgically resected oesophagus from 10 enrolled patients. Oesophageal tissues were stained using 1% methylene blue, and endo-cytoscopic images were obtained at normal and malignant areas (two areas of each) in each oesophagus. The centre of each image (4 × 10-2 mm2) was processed by computer, and the area occupied by the total nuclei in each selected field and its ratio to the entire field were calculated. Results: The mean area of the total nuclei was 0.10 × 10-2 ± 0.03 × 10-2 mm2 (range 0.05 × 10-2 to 0.18 × 10-2 mm2) in the normal group and 0.40 × 10-2 ± 0.06 × 10-2 mm2 (range 0.33 × 10-2 to 0.55 × 10-2 mm2) in the malignant group (P < 0.001). The mean ratio of total nuclei to the entire selected field was 6.4 ± 1.9% (range 3.1-11.3%) in the normal tissues and 25.3 ± 3.8% (range 20.5-34.5%) in the malignant samples (P < 0.001). Conclusions: Endo-cytoscopy system allowed automatic differentiation of normal and malignant tissues in the oesophagus, which could simplify endo-cytoscopic diagnosis. Further study will elucidate whether such analysis is applicable to inflammatory or pre-malignant epithelia in the oesophagus or other gastrointestinal organs.

Original languageEnglish
Pages (from-to)762-766
Number of pages5
JournalDigestive and Liver Disease
Volume39
Issue number8
DOIs
Publication statusPublished - 2007 Aug 1

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Keywords

  • Endo-cytoscopy
  • Image analysis
  • Oesophagus
  • Optical biopsy
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Kodashima, S., Fujishiro, M., Takubo, K., Kammori, M., Nomura, S., Kakushima, N., Muraki, Y., Goto, O., Ono, S., Kaminishi, M., & Omata, M. (2007). Ex vivo pilot study using computed analysis of endo-cytoscopic images to differentiate normal and malignant squamous cell epithelia in the oesophagus. Digestive and Liver Disease, 39(8), 762-766. https://doi.org/10.1016/j.dld.2007.03.004