Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology

Masayuki Ide, Tetsuo Ohnishi, Manabu Toyoshima, Shabeesh Balan, Motoko Maekawa, Chie Shimamoto-Mitsuyama, Yoshimi Iwayama, Hisako Ohba, Akiko Watanabe, Takashi Ishii, Norihiro Shibuya, Yuka Kimura, Yasuko Hisano, Yui Murata, Tomonori Hara, Momo Morikawa, Kenji Hashimoto, Yayoi Nozaki, Tomoko Toyota, Yuina WadaYosuke Tanaka, Tadafumi Kato, Akinori Nishi, Shigeyoshi Fujisawa, Hideyuki Okano, Masanari Itokawa, Nobutaka Hirokawa, Yasuto Kunii, Akiyoshi Kakita, Hirooki Yabe, Kazuya Iwamoto, Kohji Meno, Takuya Katagiri, Brian Dean, Kazuhiko Uchida, Hideo Kimura, Takeo Yoshikawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mice with the C3H background show greater behavioral propensity for schizophrenia, including lower prepulse inhibition (PPI), than C57BL/6 (B6) mice. To characterize as-yet-unknown pathophysiologies of schizophrenia, we undertook proteomics analysis of the brain in these strains, and detected elevated levels of Mpst, a hydrogen sulfide (H2S)/polysulfide-producing enzyme, and greater sulfide deposition in C3H than B6 mice. Mpst-deficient mice exhibited improved PPI with reduced storage sulfide levels, while Mpst-transgenic (Tg) mice showed deteriorated PPI, suggesting that “sulfide stress” may be linked to PPI impairment. Analysis of human samples demonstrated that the H2S/polysulfides production system is upregulated in schizophrenia. Mechanistically, the Mpst-Tg brain revealed dampened energy metabolism, while maternal immune activation model mice showed upregulation of genes for H2S/polysulfides production along with typical antioxidative genes, partly via epigenetic modifications. These results suggest that inflammatory/oxidative insults in early brain development result in upregulated H2S/polysulfides production as an antioxidative response, which in turn cause deficits in bioenergetic processes. Collectively, this study presents a novel aspect of the neurodevelopmental theory for schizophrenia, unraveling a role of excess H2S/polysulfides production.

Original languageEnglish
Article numbere10695
JournalEMBO Molecular Medicine
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Hydrogen Sulfide
Schizophrenia
Sulfides
Inbred C3H Mouse
Energy Metabolism
Brain
Epigenomics
Proteomics
Transgenic Mice
Genes
Up-Regulation
Mothers
polysulfide
Prepulse Inhibition
Enzymes

Keywords

  • energy metabolism
  • epigenetics
  • hydrogen sulfide and polysulfides
  • prepulse inhibition
  • proteomics

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Ide, M., Ohnishi, T., Toyoshima, M., Balan, S., Maekawa, M., Shimamoto-Mitsuyama, C., ... Yoshikawa, T. (Accepted/In press). Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. EMBO Molecular Medicine, [e10695]. https://doi.org/10.15252/emmm.201910695

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. / Ide, Masayuki; Ohnishi, Tetsuo; Toyoshima, Manabu; Balan, Shabeesh; Maekawa, Motoko; Shimamoto-Mitsuyama, Chie; Iwayama, Yoshimi; Ohba, Hisako; Watanabe, Akiko; Ishii, Takashi; Shibuya, Norihiro; Kimura, Yuka; Hisano, Yasuko; Murata, Yui; Hara, Tomonori; Morikawa, Momo; Hashimoto, Kenji; Nozaki, Yayoi; Toyota, Tomoko; Wada, Yuina; Tanaka, Yosuke; Kato, Tadafumi; Nishi, Akinori; Fujisawa, Shigeyoshi; Okano, Hideyuki; Itokawa, Masanari; Hirokawa, Nobutaka; Kunii, Yasuto; Kakita, Akiyoshi; Yabe, Hirooki; Iwamoto, Kazuya; Meno, Kohji; Katagiri, Takuya; Dean, Brian; Uchida, Kazuhiko; Kimura, Hideo; Yoshikawa, Takeo.

In: EMBO Molecular Medicine, 01.01.2019.

Research output: Contribution to journalArticle

Ide, M, Ohnishi, T, Toyoshima, M, Balan, S, Maekawa, M, Shimamoto-Mitsuyama, C, Iwayama, Y, Ohba, H, Watanabe, A, Ishii, T, Shibuya, N, Kimura, Y, Hisano, Y, Murata, Y, Hara, T, Morikawa, M, Hashimoto, K, Nozaki, Y, Toyota, T, Wada, Y, Tanaka, Y, Kato, T, Nishi, A, Fujisawa, S, Okano, H, Itokawa, M, Hirokawa, N, Kunii, Y, Kakita, A, Yabe, H, Iwamoto, K, Meno, K, Katagiri, T, Dean, B, Uchida, K, Kimura, H & Yoshikawa, T 2019, 'Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology', EMBO Molecular Medicine. https://doi.org/10.15252/emmm.201910695
Ide M, Ohnishi T, Toyoshima M, Balan S, Maekawa M, Shimamoto-Mitsuyama C et al. Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. EMBO Molecular Medicine. 2019 Jan 1. e10695. https://doi.org/10.15252/emmm.201910695
Ide, Masayuki ; Ohnishi, Tetsuo ; Toyoshima, Manabu ; Balan, Shabeesh ; Maekawa, Motoko ; Shimamoto-Mitsuyama, Chie ; Iwayama, Yoshimi ; Ohba, Hisako ; Watanabe, Akiko ; Ishii, Takashi ; Shibuya, Norihiro ; Kimura, Yuka ; Hisano, Yasuko ; Murata, Yui ; Hara, Tomonori ; Morikawa, Momo ; Hashimoto, Kenji ; Nozaki, Yayoi ; Toyota, Tomoko ; Wada, Yuina ; Tanaka, Yosuke ; Kato, Tadafumi ; Nishi, Akinori ; Fujisawa, Shigeyoshi ; Okano, Hideyuki ; Itokawa, Masanari ; Hirokawa, Nobutaka ; Kunii, Yasuto ; Kakita, Akiyoshi ; Yabe, Hirooki ; Iwamoto, Kazuya ; Meno, Kohji ; Katagiri, Takuya ; Dean, Brian ; Uchida, Kazuhiko ; Kimura, Hideo ; Yoshikawa, Takeo. / Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. In: EMBO Molecular Medicine. 2019.
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AU - Ide, Masayuki

AU - Ohnishi, Tetsuo

AU - Toyoshima, Manabu

AU - Balan, Shabeesh

AU - Maekawa, Motoko

AU - Shimamoto-Mitsuyama, Chie

AU - Iwayama, Yoshimi

AU - Ohba, Hisako

AU - Watanabe, Akiko

AU - Ishii, Takashi

AU - Shibuya, Norihiro

AU - Kimura, Yuka

AU - Hisano, Yasuko

AU - Murata, Yui

AU - Hara, Tomonori

AU - Morikawa, Momo

AU - Hashimoto, Kenji

AU - Nozaki, Yayoi

AU - Toyota, Tomoko

AU - Wada, Yuina

AU - Tanaka, Yosuke

AU - Kato, Tadafumi

AU - Nishi, Akinori

AU - Fujisawa, Shigeyoshi

AU - Okano, Hideyuki

AU - Itokawa, Masanari

AU - Hirokawa, Nobutaka

AU - Kunii, Yasuto

AU - Kakita, Akiyoshi

AU - Yabe, Hirooki

AU - Iwamoto, Kazuya

AU - Meno, Kohji

AU - Katagiri, Takuya

AU - Dean, Brian

AU - Uchida, Kazuhiko

AU - Kimura, Hideo

AU - Yoshikawa, Takeo

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