Abstract
In an attempt to further evaluate the role of neutrophil elastase (NE) in the development of emphysema, we examined the immunologic quantity of NE bound to α1-protease inhibitor (PI), the NE inhibitory activity, and the molecular pattern of α1-PI in unconcentrated bronchoalveolar lavage fluid (BALF) supernatant from 36 community-based older volunteers. They were classified into three groups: 10 current smokers with low attenuation areas (LAAs) on the lung computed tomography (CT) scans who were considered to have subclinical emphysema, 13 current smokers who had a comparable smoking history but no LAA, and 13 noncurrent smokers without LAA. The concentration of NE-α1-PI complex was significantly increased in the subjects with subclinical emphysema when compared not only with the noncurrent smokers (0.52 ± 0.10 versus 0.21 ± 0.03 SEM μg/mg albumin, p < 0.01) but also with the LAA(-) current smokers (0.52 ± 0.10 versus 0.23 ± 0.07 SEM μg/mg albumin, p < 0.01). NE inhibitory activity measured by a spectrophotometric method using methoxysuccinyl-alanyl-alanyl-prolyl-valyl-paranitroanilide did not show any significant difference between the two groups of current smokers. There was no difference in the pattern or density of native and proteolysed α1-PI bands between the three groups by Western blotting. We conclude that NE-α1-PI complex in BALF is a factor that may differentiate smokers who are potentially developing emphysema from those who are not.
| Original language | English |
|---|---|
| Pages (from-to) | 2127-2132 |
| Number of pages | 6 |
| Journal | American Journal of Respiratory and Critical Care Medicine |
| Volume | 152 |
| Issue number | 6 I |
| Publication status | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Excessive neutrophil elastase in bronchoalveolar lavage fluid in subclinical emphysema. / Yoshioka, A.; Betsuyaku, Tomoko; Nishimura, M.; Miyamoto, K.; Kondo, T.; Kawakami, Y.
In: American Journal of Respiratory and Critical Care Medicine, Vol. 152, No. 6 I, 1995, p. 2127-2132.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Excessive neutrophil elastase in bronchoalveolar lavage fluid in subclinical emphysema
AU - Yoshioka, A.
AU - Betsuyaku, Tomoko
AU - Nishimura, M.
AU - Miyamoto, K.
AU - Kondo, T.
AU - Kawakami, Y.
PY - 1995
Y1 - 1995
N2 - In an attempt to further evaluate the role of neutrophil elastase (NE) in the development of emphysema, we examined the immunologic quantity of NE bound to α1-protease inhibitor (PI), the NE inhibitory activity, and the molecular pattern of α1-PI in unconcentrated bronchoalveolar lavage fluid (BALF) supernatant from 36 community-based older volunteers. They were classified into three groups: 10 current smokers with low attenuation areas (LAAs) on the lung computed tomography (CT) scans who were considered to have subclinical emphysema, 13 current smokers who had a comparable smoking history but no LAA, and 13 noncurrent smokers without LAA. The concentration of NE-α1-PI complex was significantly increased in the subjects with subclinical emphysema when compared not only with the noncurrent smokers (0.52 ± 0.10 versus 0.21 ± 0.03 SEM μg/mg albumin, p < 0.01) but also with the LAA(-) current smokers (0.52 ± 0.10 versus 0.23 ± 0.07 SEM μg/mg albumin, p < 0.01). NE inhibitory activity measured by a spectrophotometric method using methoxysuccinyl-alanyl-alanyl-prolyl-valyl-paranitroanilide did not show any significant difference between the two groups of current smokers. There was no difference in the pattern or density of native and proteolysed α1-PI bands between the three groups by Western blotting. We conclude that NE-α1-PI complex in BALF is a factor that may differentiate smokers who are potentially developing emphysema from those who are not.
AB - In an attempt to further evaluate the role of neutrophil elastase (NE) in the development of emphysema, we examined the immunologic quantity of NE bound to α1-protease inhibitor (PI), the NE inhibitory activity, and the molecular pattern of α1-PI in unconcentrated bronchoalveolar lavage fluid (BALF) supernatant from 36 community-based older volunteers. They were classified into three groups: 10 current smokers with low attenuation areas (LAAs) on the lung computed tomography (CT) scans who were considered to have subclinical emphysema, 13 current smokers who had a comparable smoking history but no LAA, and 13 noncurrent smokers without LAA. The concentration of NE-α1-PI complex was significantly increased in the subjects with subclinical emphysema when compared not only with the noncurrent smokers (0.52 ± 0.10 versus 0.21 ± 0.03 SEM μg/mg albumin, p < 0.01) but also with the LAA(-) current smokers (0.52 ± 0.10 versus 0.23 ± 0.07 SEM μg/mg albumin, p < 0.01). NE inhibitory activity measured by a spectrophotometric method using methoxysuccinyl-alanyl-alanyl-prolyl-valyl-paranitroanilide did not show any significant difference between the two groups of current smokers. There was no difference in the pattern or density of native and proteolysed α1-PI bands between the three groups by Western blotting. We conclude that NE-α1-PI complex in BALF is a factor that may differentiate smokers who are potentially developing emphysema from those who are not.
UR - http://www.scopus.com/inward/record.url?scp=0028856719&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028856719&partnerID=8YFLogxK
M3 - Article
C2 - 8520785
AN - SCOPUS:0028856719
VL - 152
SP - 2127
EP - 2132
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 6 I
ER -