Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc

Daisuke Sakai, Yoshihiko Nakamura, Tomoko Nakai, Taishi Mishima, Shunichi Kato, Sibylle Grad, Mauro Alini, Makarand V. Risbud, Danny Chan, Kathryn S E Cheah, Ken Ichi Yamamura, Koichi Masuda, Hideyuki Okano, Kiyoshi Ando, Joji Mochida

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Despite the high prevalence of intervertebral disc disease, little is known about changes in intervertebral disc cells and their regenerative potential with ageing and intervertebral disc degeneration. Here we identify populations of progenitor cells that are Tie2 positive (Tie2+) and disialoganglioside 2 positive (GD2+), in the nucleus pulposus from mice and humans. These cells form spheroid colonies that express type II collagen and aggrecan. They are clonally multipotent and differentiated into mesenchymal lineages and induced reorganization of nucleus pulposus tissue when transplanted into non-obese diabetic/severe combined immunodeficient mice. The frequency of Tie2+ cells in tissues from patients decreases markedly with age and degeneration of the intervertebral disc, suggesting exhaustion of their capacity for regeneration. However, progenitor cells (Tie2+ GD2+) can be induced from their precursor cells (Tie2+ GD2-) under simple culture conditions. Moreover, angiopoietin-1, a ligand of Tie2, is crucial for the survival of nucleus pulposus cells. Our results offer insights for regenerative therapy and a new diagnostic standard.

Original languageEnglish
Article number1264
JournalNature Communications
Volume3
DOIs
Publication statusPublished - 2012

Fingerprint

intervertebral disks
exhaustion
Intervertebral Disc Degeneration
degeneration
Cell Aging
Stem Cells
Aging of materials
Angiopoietin-1
Tissue
Aggrecans
nuclei
Collagen Type II
cells
Ligands
mice
SCID Mice
Intervertebral Disc
Regeneration
spheroids
collagens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Sakai, D., Nakamura, Y., Nakai, T., Mishima, T., Kato, S., Grad, S., ... Mochida, J. (2012). Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc. Nature Communications, 3, [1264]. https://doi.org/10.1038/ncomms2226

Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc. / Sakai, Daisuke; Nakamura, Yoshihiko; Nakai, Tomoko; Mishima, Taishi; Kato, Shunichi; Grad, Sibylle; Alini, Mauro; Risbud, Makarand V.; Chan, Danny; Cheah, Kathryn S E; Yamamura, Ken Ichi; Masuda, Koichi; Okano, Hideyuki; Ando, Kiyoshi; Mochida, Joji.

In: Nature Communications, Vol. 3, 1264, 2012.

Research output: Contribution to journalArticle

Sakai, D, Nakamura, Y, Nakai, T, Mishima, T, Kato, S, Grad, S, Alini, M, Risbud, MV, Chan, D, Cheah, KSE, Yamamura, KI, Masuda, K, Okano, H, Ando, K & Mochida, J 2012, 'Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc', Nature Communications, vol. 3, 1264. https://doi.org/10.1038/ncomms2226
Sakai, Daisuke ; Nakamura, Yoshihiko ; Nakai, Tomoko ; Mishima, Taishi ; Kato, Shunichi ; Grad, Sibylle ; Alini, Mauro ; Risbud, Makarand V. ; Chan, Danny ; Cheah, Kathryn S E ; Yamamura, Ken Ichi ; Masuda, Koichi ; Okano, Hideyuki ; Ando, Kiyoshi ; Mochida, Joji. / Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc. In: Nature Communications. 2012 ; Vol. 3.
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