Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes

Kyle L. Fort; Michiel Van De Waterbeemd; Dmitriy Boll; Maria Reinhardt-Szyba; Mikhail E. Belov; Eita Sasaki; Reinhard Zschoche; Donald Hilvert; Alexander A. Makarov; Albert J.R. Heck

doi:10.1039/c7an01629h

Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes

Kyle L. Fort, Michiel Van De Waterbeemd, Dmitriy Boll, Maria Reinhardt-Szyba, Mikhail E. Belov, Eita Sasaki, Reinhard Zschoche, Donald Hilvert, Alexander A. Makarov, Albert J.R. Heck

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Native mass spectrometry can provide insight into the structure of macromolecular biological systems. As analytes under investigation get larger and more complex, instrument capabilities need to be advanced. Herein, modifications to an Orbitrap Q Exactive Plus mass spectrometer that increase signal intensity, mass resolution, and maximum m/z measurable are described.

Original language	English
Pages (from-to)	100-105
Number of pages	6
Journal	Analyst
Volume	143
Issue number	1
DOIs	https://doi.org/10.1039/c7an01629h
Publication status	Published - 2018 Jan 7
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/c7an01629h

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title = "Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes",

abstract = "Native mass spectrometry can provide insight into the structure of macromolecular biological systems. As analytes under investigation get larger and more complex, instrument capabilities need to be advanced. Herein, modifications to an Orbitrap Q Exactive Plus mass spectrometer that increase signal intensity, mass resolution, and maximum m/z measurable are described.",

author = "Fort, {Kyle L.} and {Van De Waterbeemd}, Michiel and Dmitriy Boll and Maria Reinhardt-Szyba and Belov, {Mikhail E.} and Eita Sasaki and Reinhard Zschoche and Donald Hilvert and Makarov, {Alexander A.} and Heck, {Albert J.R.}",

note = "Funding Information: We acknowledge the support from the Netherlands Organization for Scientific Research (NWO) funding the large-scale proteomics facility Proteins@Work (project 184.032.201) embedded in the Netherlands Proteomics Centre. M. W. and A. J. R. H. are additionally supported by a Projectruimte grant (12PR3303-2) from Fundamenteel Onderzoek der Materie (FOM). K. F., A. M. and A. J. R. H. acknowledge additional support through the European Union Horizon 2020 programme FET-OPEN project MSmed, Project 686547. A. J. R. H. acknowledge further support by the NWO TOP-Punt Grant 718.015.003. Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

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doi = "10.1039/c7an01629h",

language = "English",

volume = "143",

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T1 - Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes

AU - Fort, Kyle L.

AU - Van De Waterbeemd, Michiel

AU - Boll, Dmitriy

AU - Reinhardt-Szyba, Maria

AU - Belov, Mikhail E.

AU - Sasaki, Eita

AU - Zschoche, Reinhard

AU - Hilvert, Donald

AU - Makarov, Alexander A.

AU - Heck, Albert J.R.

N1 - Funding Information: We acknowledge the support from the Netherlands Organization for Scientific Research (NWO) funding the large-scale proteomics facility Proteins@Work (project 184.032.201) embedded in the Netherlands Proteomics Centre. M. W. and A. J. R. H. are additionally supported by a Projectruimte grant (12PR3303-2) from Fundamenteel Onderzoek der Materie (FOM). K. F., A. M. and A. J. R. H. acknowledge additional support through the European Union Horizon 2020 programme FET-OPEN project MSmed, Project 686547. A. J. R. H. acknowledge further support by the NWO TOP-Punt Grant 718.015.003. Publisher Copyright: © 2018 The Royal Society of Chemistry.

PY - 2018/1/7

Y1 - 2018/1/7

N2 - Native mass spectrometry can provide insight into the structure of macromolecular biological systems. As analytes under investigation get larger and more complex, instrument capabilities need to be advanced. Herein, modifications to an Orbitrap Q Exactive Plus mass spectrometer that increase signal intensity, mass resolution, and maximum m/z measurable are described.

AB - Native mass spectrometry can provide insight into the structure of macromolecular biological systems. As analytes under investigation get larger and more complex, instrument capabilities need to be advanced. Herein, modifications to an Orbitrap Q Exactive Plus mass spectrometer that increase signal intensity, mass resolution, and maximum m/z measurable are described.

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Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes

Abstract

ASJC Scopus subject areas

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