CERN 's new particle accelerator Linac4 is part of the upgrade of the LHC accelerator chain. Linac4 is required to deliver 160 MeV H- beam to improve the beam brightness and luminosity in the Large Hadron Collider (LHC). The Linac4 H- source must deliver 40-50 mA, 45 keV H- beam in the RFQ acceptance. Since the RF power coupled to the H- source plasma is one of the important parameters that determines the quality of the H- beam, the experimental investigation of the dependence of the load impedance on the operational parameters is mandatory. In this study, we have measured the impedance of the H- source plasma varying the RF power coupled to the plasma and the condition of the hydrogen gas. Also, optical emission spectroscopy (OES) measurements have been carried out simultaneously with the impedance measurement in order to determine the plasma parameters. The determination of the plasma parameters allows us to compare the experimental results with the analytic model of the plasma parameters, which is useful to discuss the results from a physical point of view.