Experimental investigation of various administration methods of gentamicin. Comparison of the effect of gentamicin on phagocytosis

Y. Iwasaki, N. Yamashita, K. Jozaki, M. Tojo, Y. Sato, S. Iwata, H. Akita, K. Sunakawa, T. Oikawa, M. Osano

Research output: Contribution to journalArticle

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Abstract

We compared the efficacy of gentamicin (GM) intravenously or intramuscularly administered or intravenously infused over 45 minutes by the experimental system in rabbits using diffusion chambers. Serum concentrations of GM obtained with the 3 administration methods were different, while the peak values after intramuscular injection and intravenous drip infusion were similar. Similar concentration time curves of GM in the chambers were revealed after intramuscular injection and intravenous infusion. No marked difference in the 3 administration methods was seen in the effects on the growth of P. aeruginosa in the chamber. Viable bacterial number in the chambers decreased when the GM concentration in the chamber was 4 ~ 5 times of MIC, and thereafter regrowth was observed after the decrease of GM concentration in the chambers to 2 ~ 3 times of MIC. About 8 hours were required for growth to the base line value. In the leucocyte containing chambers, viable bacterial number similarly decreased, but the rate of regrowth was slow. About 12 hours were required for growth to the base line value. The regrowth rate in the GM containing chamber was similar to that in the antibiotic free chamber. From this result, it is suggested that, when the antibiotic concentration in the chamber is 1 ~ 2 times of MIC, antibiotic does not show the growth suppressive effect and the activating effect on phagocytosis of leucocytes in the environment closed to the practical pathological condition such as this model. For the patients with qualitative or quantitative abnormal changes of phagocyte, short interval of drug administration might be needed. For this purpose, intravenous drip infusion under monitoring of serum concentration is more suitable than intramuscular injection, which might be accompanied by severe pain and contracture of the injected muscle.

Original languageEnglish
Pages (from-to)547-551
Number of pages5
JournalJapanese Journal of Antibiotics
Volume36
Issue number3
Publication statusPublished - 1983

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Gentamicins
Phagocytosis
Intravenous Infusions
Intramuscular Injections
Growth
Anti-Bacterial Agents
Leukocytes
Contracture
Phagocytes
Serum
Rabbits
Pain
Muscles
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

Iwasaki, Y., Yamashita, N., Jozaki, K., Tojo, M., Sato, Y., Iwata, S., ... Osano, M. (1983). Experimental investigation of various administration methods of gentamicin. Comparison of the effect of gentamicin on phagocytosis. Japanese Journal of Antibiotics, 36(3), 547-551.

Experimental investigation of various administration methods of gentamicin. Comparison of the effect of gentamicin on phagocytosis. / Iwasaki, Y.; Yamashita, N.; Jozaki, K.; Tojo, M.; Sato, Y.; Iwata, S.; Akita, H.; Sunakawa, K.; Oikawa, T.; Osano, M.

In: Japanese Journal of Antibiotics, Vol. 36, No. 3, 1983, p. 547-551.

Research output: Contribution to journalArticle

Iwasaki, Y, Yamashita, N, Jozaki, K, Tojo, M, Sato, Y, Iwata, S, Akita, H, Sunakawa, K, Oikawa, T & Osano, M 1983, 'Experimental investigation of various administration methods of gentamicin. Comparison of the effect of gentamicin on phagocytosis', Japanese Journal of Antibiotics, vol. 36, no. 3, pp. 547-551.
Iwasaki, Y. ; Yamashita, N. ; Jozaki, K. ; Tojo, M. ; Sato, Y. ; Iwata, S. ; Akita, H. ; Sunakawa, K. ; Oikawa, T. ; Osano, M. / Experimental investigation of various administration methods of gentamicin. Comparison of the effect of gentamicin on phagocytosis. In: Japanese Journal of Antibiotics. 1983 ; Vol. 36, No. 3. pp. 547-551.
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