Experimental parathyroid transplantation

Human parathyroid grafts survived and functioned in mice treated with anti-CD4 monoclonal antibody

M. Niimi, M. Takashina, H. Takami, N. Shirasugi, K. Hamano, K. Esato, K. Matsumoto, Y. Ikeda, T. Shatari, S. Kodaira, Kaori Kameyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Permanent hypoparathyroidism is one of the most difficult of all endocrine disorders to treat medically. To examine the possibility that xenotransplantation can be used to treat hypoparathyroidism, human parathyroid tissues were transplanted into mice. Human parathyroid tissue was taken from specimens excised from patients with hyperparathyroidism. Fresh human parathyroid tissue was implanted under kidney capsule of CBA (H2(k)) mice. Some mice were treated intraperitoneally with depleting anti-CD4 monoclonal antibody (mAb, YTA 3.1, 100 μg/dose, days -1, 0, 1, 2, 3, and 5). Mice were killed 30 days after transplantation. Survival of parathyroid grafts was examined microscopically and human parathyroid hormone in serum was measured by ELISA. All parathyroid grafts survived under kidney capsule and human parathyroid hormone was strongly detected in serum (621 ± 576 pg/mL) when recipients were treated with short-course treatment of anti-CD4 mAb. Conversely, no parathyroid tissue was seen microscopically in any recipient mice without anti-CD4 mAb treatment. Human parathyroid hormone was undetectable by ELISA in naive mice and mice transplanted with human parathyroid tissue without short-course treatment of anti-CD4 mAb. Xenogeneic human parathyroid tissue survived and functioned in mice treated with Short- course treatment of anti-CD4 mAb. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

Original languageEnglish
JournalBiomedicine and Pharmacotherapy
Volume54
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2000 Jun

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Transplantation
Monoclonal Antibodies
Transplants
Hypoparathyroidism
Capsules
Enzyme-Linked Immunosorbent Assay
Kidney
Heterologous Transplantation
Hyperparathyroidism
Graft Survival
Therapeutics
Serum
human PTH protein

Keywords

  • Anti-CD4 mAb
  • Parathyroid
  • Xenotransplantation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Experimental parathyroid transplantation : Human parathyroid grafts survived and functioned in mice treated with anti-CD4 monoclonal antibody. / Niimi, M.; Takashina, M.; Takami, H.; Shirasugi, N.; Hamano, K.; Esato, K.; Matsumoto, K.; Ikeda, Y.; Shatari, T.; Kodaira, S.; Kameyama, Kaori.

In: Biomedicine and Pharmacotherapy, Vol. 54, No. SUPPL. 1, 06.2000.

Research output: Contribution to journalArticle

Niimi, M. ; Takashina, M. ; Takami, H. ; Shirasugi, N. ; Hamano, K. ; Esato, K. ; Matsumoto, K. ; Ikeda, Y. ; Shatari, T. ; Kodaira, S. ; Kameyama, Kaori. / Experimental parathyroid transplantation : Human parathyroid grafts survived and functioned in mice treated with anti-CD4 monoclonal antibody. In: Biomedicine and Pharmacotherapy. 2000 ; Vol. 54, No. SUPPL. 1.
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