Experimental study of intraparenchymal fibrinogen and topical thrombin to seal pleural defects

Jun Yamamoto, Mitsutomo Kohno, Naoko Izawa, Toshinari Ema, Tai Hato, Ikuo Kamiyama, Takashi Ohtsuka, Masazumi Watanabe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: Fibrin sealants are used to close surgical pleural defects, but may detach, causing a postoperative air-leak. We investigated a new means of applying fibrin glue for closing pleural defects. Methods: Pleural defects (10-mm and 4-mm diameters, respectively) were created in swine and rats via thoracotomy. They were sealed by a) injection of a fibrinogen solution into the lung parenchyma after instillation of a thrombin solution onto the pleural defect (group A), b) fibrinogen and thrombin spray (group B), c) fibrinogen instillation and a thrombin-dipped polyglycolic acid sheet (group C), or d) fibrin glue-coated collagen fleece (group D). Resistance to airway pressure was compared and the sealed areas were histologically examined. Results: In group A, the minimum seal-breaking airway pressure was consistently > 40 cmH2O, versus 37.2 ± 3.6 cmH2O in group B, 37.2 ± 4.0 cmH2O in group C, and 39.0 ± 1.7 cmH2O in group D, which was statistically significant. Histologically, the fibrin layer infiltrated the lung parenchyma and covered the defect in group A, but not in the other groups. Conclusions: The intraparenchymal injection of fibrinogen combined with instillation of thrombin created an effective fibrin layer associated with early pleural regeneration that reliably prevented pleural air leaks.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalTokai Journal of Experimental and Clinical Medicine
Volume41
Issue number4
Publication statusPublished - 2016 Dec 1

Fingerprint

Thrombin
Fibrinogen
Fibrin Tissue Adhesive
Fibrin
Air
Polyglycolic Acid
Pressure
Lung
Injections
Airway Resistance
Thoracotomy
Regeneration
Swine
Collagen

Keywords

  • Air leak
  • Fibrin glue
  • Pleural defect repair
  • Sealant

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yamamoto, J., Kohno, M., Izawa, N., Ema, T., Hato, T., Kamiyama, I., ... Watanabe, M. (2016). Experimental study of intraparenchymal fibrinogen and topical thrombin to seal pleural defects. Tokai Journal of Experimental and Clinical Medicine, 41(4), 185-189.

Experimental study of intraparenchymal fibrinogen and topical thrombin to seal pleural defects. / Yamamoto, Jun; Kohno, Mitsutomo; Izawa, Naoko; Ema, Toshinari; Hato, Tai; Kamiyama, Ikuo; Ohtsuka, Takashi; Watanabe, Masazumi.

In: Tokai Journal of Experimental and Clinical Medicine, Vol. 41, No. 4, 01.12.2016, p. 185-189.

Research output: Contribution to journalArticle

Yamamoto, J, Kohno, M, Izawa, N, Ema, T, Hato, T, Kamiyama, I, Ohtsuka, T & Watanabe, M 2016, 'Experimental study of intraparenchymal fibrinogen and topical thrombin to seal pleural defects', Tokai Journal of Experimental and Clinical Medicine, vol. 41, no. 4, pp. 185-189.
Yamamoto, Jun ; Kohno, Mitsutomo ; Izawa, Naoko ; Ema, Toshinari ; Hato, Tai ; Kamiyama, Ikuo ; Ohtsuka, Takashi ; Watanabe, Masazumi. / Experimental study of intraparenchymal fibrinogen and topical thrombin to seal pleural defects. In: Tokai Journal of Experimental and Clinical Medicine. 2016 ; Vol. 41, No. 4. pp. 185-189.
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