Human physiology dictates that the sole temperature drops by touching a cold floor in a poor insulation, whereupon the peripheral temperature receptor stimulates the sympathetic nervous system, leading to an increase in Blood Pressure (BP) due to vasoconstriction. Furthermore, flooring material affects perceived thermal sensation. This study aims to analyse the relationship between floor temperature, flooring material and BP. 10 healthy male university students and 13 middle-aged men participated in this study, which was conducted in October 2015. The floor temperature was 21.2 °C in a well-insulated room compared with 17.2 °C in an uninsulated. In the younger, the average BP was insensitive to floor temperature. In the older, however, BP rose by about 3.7 mmHg with solid wood flooring and by about 7.5 mmHg with vinyl after an hour in the uninsulated. Despite this, a significant difference in BP elevation was not confirmed between floor materials.