Experimental transplantation study for possible transformation of bone marrow cells in the mouse placenta

K. Kakui, Hiroshi Itoh, N. Sagawa, S. Yura, M. Takemura, M. Kawamura, S. Fujii

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of the present study is to establish a mouse model of the transplantation of bone marrow cells into the placenta in mid-gestation. The mononuclear fraction of bone marrow cells was isolated by Ficoll gradient centrifugation from the femur bones of C57BL/6 green fluorescent protein (GFP) gene transgenic (Tg) mice. After intraperitoneal injection of pentobarbital sodium, the abdominal cavities of pregnant non-Tg (C57BL/6 or ICR) mice were opened at 9.5 days postcoitum (dpc). The mononuclear fraction of bone marrow cells from Tg mice (3-5 × 105 cells/3 μl) was directly injected into the placental portion of the pregnant uterus, at a depth of approximately 3 mm, using a 31-gauge injector. The placenta was sampled at 14.5 dpc. Confocal laser scanning microscopic analysis of the serial sections of the sampled placenta (150-250 sections/placenta) was carried out to detect GFP-positive cells and to assess immunostaining for cytokeratin, CD34, p57Kip2 and prolactin. Most pregnant mice survived until sampling of the placenta at 14.5-18.5 dpc (88.9% for C57BL6 and 100% for ICR). The survival rate of fetuses from mice in which the placenta was transplanted with GFP-positive bone marrow cells was approximately 50%. A small population (0.154%) of injected bone marrow cells was retained in the placental tissue. Immunohistochemically, cytokeratin, CD34 and p57Kip2 were positively stained in 0.062%, 4.5% and 2.1% of GFP-positive cells, respectively, while prolactin was not positive in any of the cells examined. GFP-positive bone marrow cells were successfully transplanted to the murine placenta. Future investigations of the specific antigens in bone marrow cells retained in the placenta may enable a better understanding of the local regulation of placental development.

Original languageEnglish
Pages (from-to)678-685
Number of pages8
JournalPlacenta
Volume26
Issue number8-9
DOIs
Publication statusPublished - 2005 Sep
Externally publishedYes

Fingerprint

Bone Marrow Cells
Placenta
Transplantation
Green Fluorescent Proteins
Keratins
Prolactin
Transgenic Mice
Retained Placenta
Placentation
Ficoll
Inbred ICR Mouse
Abdominal Cavity
Pentobarbital
Intraperitoneal Injections
Bone Marrow Transplantation
Centrifugation
Femur
Uterus
Lasers
Fetus

Keywords

  • Bone marrow cells
  • Development
  • Placenta
  • Pregnancy
  • Transplantation

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Experimental transplantation study for possible transformation of bone marrow cells in the mouse placenta. / Kakui, K.; Itoh, Hiroshi; Sagawa, N.; Yura, S.; Takemura, M.; Kawamura, M.; Fujii, S.

In: Placenta, Vol. 26, No. 8-9, 09.2005, p. 678-685.

Research output: Contribution to journalArticle

Kakui, K. ; Itoh, Hiroshi ; Sagawa, N. ; Yura, S. ; Takemura, M. ; Kawamura, M. ; Fujii, S. / Experimental transplantation study for possible transformation of bone marrow cells in the mouse placenta. In: Placenta. 2005 ; Vol. 26, No. 8-9. pp. 678-685.
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