Expression analysis and evolutionary conservation of the mouse germ cell-specific D6Mm5e gene

Nelson A. Arango, Tiffany T. Huang, Akihiro Fujino, Rafael Pieretti-Vanmarcke, Patricia K. Donahoe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During our search for genes required for gonadal development and function in the mouse, we identified D6Mm5e (DNA segment, Chr 6. Miriam Meisler 5, expressed), a gene with an expression pattern highly restricted to the embryonic ovary and the postnatal testis. Based on RT-PCR, Northern blot, and in situ hybridization analyses, we show that D6Mm5e is expressed in the germ cells of the female embryo upon their initial entry into meiosis, and in male germ cells during the last stages of spermatogenesis. Two transcripts are detected in the gonads resulting from the alternative splicing of exon 8. This splicing event does not introduce a frame shift, and creates an mRNA product that uses the same stop codon as the longer transcript. Although D6Mm5e does not belong to any known protein family and does not contain any known protein signature motifs, the high level of evolutionary conservation and the cellular and temporal expression suggest that D6Mm5e may have a role in male and female gametogenesis. Here we report the gonad-restricted mRNA expression profile of D6Mm5e in the mouse, and the evolutionary conservation of its amino acid sequence.

Original languageEnglish
Pages (from-to)2613-2619
Number of pages7
JournalDevelopmental Dynamics
Volume235
Issue number9
DOIs
Publication statusPublished - 2006 Sep
Externally publishedYes

Fingerprint

Gonads
Germ Cells
Gametogenesis
Messenger RNA
Amino Acid Motifs
Terminator Codon
Meiosis
Alternative Splicing
Spermatogenesis
Northern Blotting
Genes
In Situ Hybridization
Testis
Amino Acid Sequence
Ovary
Exons
Embryonic Structures
Gene Expression
Polymerase Chain Reaction
DNA

Keywords

  • Gametogenesis
  • Meiosis
  • Ovary development
  • Sexual differentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Arango, N. A., Huang, T. T., Fujino, A., Pieretti-Vanmarcke, R., & Donahoe, P. K. (2006). Expression analysis and evolutionary conservation of the mouse germ cell-specific D6Mm5e gene. Developmental Dynamics, 235(9), 2613-2619. https://doi.org/10.1002/dvdy.20907

Expression analysis and evolutionary conservation of the mouse germ cell-specific D6Mm5e gene. / Arango, Nelson A.; Huang, Tiffany T.; Fujino, Akihiro; Pieretti-Vanmarcke, Rafael; Donahoe, Patricia K.

In: Developmental Dynamics, Vol. 235, No. 9, 09.2006, p. 2613-2619.

Research output: Contribution to journalArticle

Arango, NA, Huang, TT, Fujino, A, Pieretti-Vanmarcke, R & Donahoe, PK 2006, 'Expression analysis and evolutionary conservation of the mouse germ cell-specific D6Mm5e gene', Developmental Dynamics, vol. 235, no. 9, pp. 2613-2619. https://doi.org/10.1002/dvdy.20907
Arango, Nelson A. ; Huang, Tiffany T. ; Fujino, Akihiro ; Pieretti-Vanmarcke, Rafael ; Donahoe, Patricia K. / Expression analysis and evolutionary conservation of the mouse germ cell-specific D6Mm5e gene. In: Developmental Dynamics. 2006 ; Vol. 235, No. 9. pp. 2613-2619.
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