Expression and characterization of endothelial cell receptors in hematopietic cells

Isao Hamaauchi, Hitoshi Nisflio, Tomohisa Inada, Aisuo Sato, Nobuyuki Takakura, Atsushi Iwama, Junto Suda, Toshio Suda

Research output: Contribution to journalArticle

Abstract

Receptor tyrosine kinases(RTK), TIE, TEK, FLT and FLK are reported to be expressed in vascular endothelial cells. Vascular endothelial cells and hematopoietic cells are derived from the common progenitors, hemangioblasts. Thus, it is interesting to see the expression and function of endothelial cell RTK on hematopoietic cells. We have established monoclonal antibodies against murlne and human RTK and examined their expression in hematopoietic cells. TIE and TEK belong to unclassified RTK and they showed high homology. Both genes were expressed in a similar fashion in adult tissues and primitive hematopoietic cells. Flow cytometry shows that TIE and TEK were mainly expressed in hematopoietic stem cell fractions; Lin-Kit+ fraction of bone marrow cells and fetal liver cells. In human bone marrow cells, TIE was expressed on hematopoietic progenitors including primitive stem cells, CD34highCD38- cells, while TEK was expressed in CD34kw cells. FLT and FLK are receptors for VEGF and belong to class V.FLT was expressed on TER1l9-positive erythroblasts in fetal liver cells and FLK-1 was expressed on AGM region. We have establshed the cell culture system of AGM cells using stromal cells.Cultured AGM cells contained a significant number of Lin-Kit+Sca-t+ cells, which were able to reconstitute hematopoiesis in lethally irradiated mice. We would like to discuss about the expression of RTK on these cells.

Original languageEnglish
Pages (from-to)804
Number of pages1
JournalExperimental Hematology
Volume25
Issue number8
Publication statusPublished - 1997

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Endothelial Cells
Receptor Protein-Tyrosine Kinases
TIE Receptors
Bone Marrow Cells
Proto-Oncogene Proteins c-hck
Hemangioblasts
Vascular Endothelial Growth Factor Receptor-1
Erythroblasts
Liver
Hematopoiesis
Stromal Cells
Hematopoietic Stem Cells
Cultured Cells
Flow Cytometry
Stem Cells
Cell Culture Techniques
Monoclonal Antibodies
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Hamaauchi, I., Nisflio, H., Inada, T., Sato, A., Takakura, N., Iwama, A., ... Suda, T. (1997). Expression and characterization of endothelial cell receptors in hematopietic cells. Experimental Hematology, 25(8), 804.

Expression and characterization of endothelial cell receptors in hematopietic cells. / Hamaauchi, Isao; Nisflio, Hitoshi; Inada, Tomohisa; Sato, Aisuo; Takakura, Nobuyuki; Iwama, Atsushi; Suda, Junto; Suda, Toshio.

In: Experimental Hematology, Vol. 25, No. 8, 1997, p. 804.

Research output: Contribution to journalArticle

Hamaauchi, I, Nisflio, H, Inada, T, Sato, A, Takakura, N, Iwama, A, Suda, J & Suda, T 1997, 'Expression and characterization of endothelial cell receptors in hematopietic cells', Experimental Hematology, vol. 25, no. 8, pp. 804.
Hamaauchi I, Nisflio H, Inada T, Sato A, Takakura N, Iwama A et al. Expression and characterization of endothelial cell receptors in hematopietic cells. Experimental Hematology. 1997;25(8):804.
Hamaauchi, Isao ; Nisflio, Hitoshi ; Inada, Tomohisa ; Sato, Aisuo ; Takakura, Nobuyuki ; Iwama, Atsushi ; Suda, Junto ; Suda, Toshio. / Expression and characterization of endothelial cell receptors in hematopietic cells. In: Experimental Hematology. 1997 ; Vol. 25, No. 8. pp. 804.
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