Expression and distribution of brain natriuretic peptide in human right atria

Kiyoshi Doyama, Manabu Fukumoto, Genzou Takemura, Masaru Tanaka, Teiji Oda, Koji Hasegawa, Tsukasa Inada, Seiji Ohtani, Takako Fujiwara, Hiroshi Itoh, Kazuwa Nakao, Shigetake Sasayama, Hisayoshi Fujiwara

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Objectives. We investigated expression of brain natriuretic peptide (BNP) as well as atrial natriuretic peptide (ANP) and their genes in human right atria. Their relations with atrial pressure were also examined. Background. The BNP plays a roll in electrolyte-fluid homeostasis such as ANP. The tissue level is reported to be elevated in the failing ventricles. However, expression and transmural distribution of BNP in the atria remain unclear. Methods. Expression of ANP and BNP was immunohistochemically investigated in the right atrial (RA) specimens from 21 patients who had undergone cardiac surgery. The mRNA of specimens were quantitatively measured by Northern blot analysis and also evaluated by in situ hybridization. In addition, plasma levels of ANP and BNP were measured in the patients. Results. The BNP immunoreactivity was diffusely seen in RA tissue of patients with mean RA pressure (mRAP) of 5 mm Hg or more, but it was noted only in the subendocardial half of the atria of those with mRAP less than 5 mm Hg. There was a significant correlation between the incidence of BNP-positive myocytes and mRAP (r = 0.850, p < 0.0001). Conversely, ANP-positive myocytes were found diffusely in all cases. In Northern blot analysis, the mRNAs levels of ANP and BNP in the atrial tissue were positively correlated with the mRAP (ANP, p = 0.775, p < 0.005 and BNP, p = 0.771, p < 0.005). In situ hybridization confirmed these findings. The mRNA levels were significantly correlated to each other (r = 0.845, p < 0.0002). Plasma ANP and BNP levels were elevated in the patients compared with that in controls; however, none were significantly correlated with the mRAP. Conclusions. Expression of BNP and BNP mRNA is augmented in the atria with increased pressure, and distributed predominantly in the subendocardial side. The level of BNP mRNA was well correlated with that of ANP mRNA. Thus, these two genes might be commonly regulated in response to atrial pressure.

Original languageEnglish
Pages (from-to)1832-1838
Number of pages7
JournalJournal of the American College of Cardiology
Volume32
Issue number7
DOIs
Publication statusPublished - 1998 Dec
Externally publishedYes

Fingerprint

Brain Natriuretic Peptide
Heart Atria
Atrial Natriuretic Factor
Messenger RNA
Atrial Pressure
Pressure
Northern Blotting
Muscle Cells
In Situ Hybridization
Electrolytes
Genes
Thoracic Surgery
Homeostasis

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Doyama, K., Fukumoto, M., Takemura, G., Tanaka, M., Oda, T., Hasegawa, K., ... Fujiwara, H. (1998). Expression and distribution of brain natriuretic peptide in human right atria. Journal of the American College of Cardiology, 32(7), 1832-1838. https://doi.org/10.1016/S0735-1097(98)00494-X

Expression and distribution of brain natriuretic peptide in human right atria. / Doyama, Kiyoshi; Fukumoto, Manabu; Takemura, Genzou; Tanaka, Masaru; Oda, Teiji; Hasegawa, Koji; Inada, Tsukasa; Ohtani, Seiji; Fujiwara, Takako; Itoh, Hiroshi; Nakao, Kazuwa; Sasayama, Shigetake; Fujiwara, Hisayoshi.

In: Journal of the American College of Cardiology, Vol. 32, No. 7, 12.1998, p. 1832-1838.

Research output: Contribution to journalArticle

Doyama, K, Fukumoto, M, Takemura, G, Tanaka, M, Oda, T, Hasegawa, K, Inada, T, Ohtani, S, Fujiwara, T, Itoh, H, Nakao, K, Sasayama, S & Fujiwara, H 1998, 'Expression and distribution of brain natriuretic peptide in human right atria', Journal of the American College of Cardiology, vol. 32, no. 7, pp. 1832-1838. https://doi.org/10.1016/S0735-1097(98)00494-X
Doyama, Kiyoshi ; Fukumoto, Manabu ; Takemura, Genzou ; Tanaka, Masaru ; Oda, Teiji ; Hasegawa, Koji ; Inada, Tsukasa ; Ohtani, Seiji ; Fujiwara, Takako ; Itoh, Hiroshi ; Nakao, Kazuwa ; Sasayama, Shigetake ; Fujiwara, Hisayoshi. / Expression and distribution of brain natriuretic peptide in human right atria. In: Journal of the American College of Cardiology. 1998 ; Vol. 32, No. 7. pp. 1832-1838.
@article{8999a99d3206484ab3822cc830299e5b,
title = "Expression and distribution of brain natriuretic peptide in human right atria",
abstract = "Objectives. We investigated expression of brain natriuretic peptide (BNP) as well as atrial natriuretic peptide (ANP) and their genes in human right atria. Their relations with atrial pressure were also examined. Background. The BNP plays a roll in electrolyte-fluid homeostasis such as ANP. The tissue level is reported to be elevated in the failing ventricles. However, expression and transmural distribution of BNP in the atria remain unclear. Methods. Expression of ANP and BNP was immunohistochemically investigated in the right atrial (RA) specimens from 21 patients who had undergone cardiac surgery. The mRNA of specimens were quantitatively measured by Northern blot analysis and also evaluated by in situ hybridization. In addition, plasma levels of ANP and BNP were measured in the patients. Results. The BNP immunoreactivity was diffusely seen in RA tissue of patients with mean RA pressure (mRAP) of 5 mm Hg or more, but it was noted only in the subendocardial half of the atria of those with mRAP less than 5 mm Hg. There was a significant correlation between the incidence of BNP-positive myocytes and mRAP (r = 0.850, p < 0.0001). Conversely, ANP-positive myocytes were found diffusely in all cases. In Northern blot analysis, the mRNAs levels of ANP and BNP in the atrial tissue were positively correlated with the mRAP (ANP, p = 0.775, p < 0.005 and BNP, p = 0.771, p < 0.005). In situ hybridization confirmed these findings. The mRNA levels were significantly correlated to each other (r = 0.845, p < 0.0002). Plasma ANP and BNP levels were elevated in the patients compared with that in controls; however, none were significantly correlated with the mRAP. Conclusions. Expression of BNP and BNP mRNA is augmented in the atria with increased pressure, and distributed predominantly in the subendocardial side. The level of BNP mRNA was well correlated with that of ANP mRNA. Thus, these two genes might be commonly regulated in response to atrial pressure.",
author = "Kiyoshi Doyama and Manabu Fukumoto and Genzou Takemura and Masaru Tanaka and Teiji Oda and Koji Hasegawa and Tsukasa Inada and Seiji Ohtani and Takako Fujiwara and Hiroshi Itoh and Kazuwa Nakao and Shigetake Sasayama and Hisayoshi Fujiwara",
year = "1998",
month = "12",
doi = "10.1016/S0735-1097(98)00494-X",
language = "English",
volume = "32",
pages = "1832--1838",
journal = "Journal of the American College of Cardiology",
issn = "0735-1097",
publisher = "Elsevier USA",
number = "7",

}

TY - JOUR

T1 - Expression and distribution of brain natriuretic peptide in human right atria

AU - Doyama, Kiyoshi

AU - Fukumoto, Manabu

AU - Takemura, Genzou

AU - Tanaka, Masaru

AU - Oda, Teiji

AU - Hasegawa, Koji

AU - Inada, Tsukasa

AU - Ohtani, Seiji

AU - Fujiwara, Takako

AU - Itoh, Hiroshi

AU - Nakao, Kazuwa

AU - Sasayama, Shigetake

AU - Fujiwara, Hisayoshi

PY - 1998/12

Y1 - 1998/12

N2 - Objectives. We investigated expression of brain natriuretic peptide (BNP) as well as atrial natriuretic peptide (ANP) and their genes in human right atria. Their relations with atrial pressure were also examined. Background. The BNP plays a roll in electrolyte-fluid homeostasis such as ANP. The tissue level is reported to be elevated in the failing ventricles. However, expression and transmural distribution of BNP in the atria remain unclear. Methods. Expression of ANP and BNP was immunohistochemically investigated in the right atrial (RA) specimens from 21 patients who had undergone cardiac surgery. The mRNA of specimens were quantitatively measured by Northern blot analysis and also evaluated by in situ hybridization. In addition, plasma levels of ANP and BNP were measured in the patients. Results. The BNP immunoreactivity was diffusely seen in RA tissue of patients with mean RA pressure (mRAP) of 5 mm Hg or more, but it was noted only in the subendocardial half of the atria of those with mRAP less than 5 mm Hg. There was a significant correlation between the incidence of BNP-positive myocytes and mRAP (r = 0.850, p < 0.0001). Conversely, ANP-positive myocytes were found diffusely in all cases. In Northern blot analysis, the mRNAs levels of ANP and BNP in the atrial tissue were positively correlated with the mRAP (ANP, p = 0.775, p < 0.005 and BNP, p = 0.771, p < 0.005). In situ hybridization confirmed these findings. The mRNA levels were significantly correlated to each other (r = 0.845, p < 0.0002). Plasma ANP and BNP levels were elevated in the patients compared with that in controls; however, none were significantly correlated with the mRAP. Conclusions. Expression of BNP and BNP mRNA is augmented in the atria with increased pressure, and distributed predominantly in the subendocardial side. The level of BNP mRNA was well correlated with that of ANP mRNA. Thus, these two genes might be commonly regulated in response to atrial pressure.

AB - Objectives. We investigated expression of brain natriuretic peptide (BNP) as well as atrial natriuretic peptide (ANP) and their genes in human right atria. Their relations with atrial pressure were also examined. Background. The BNP plays a roll in electrolyte-fluid homeostasis such as ANP. The tissue level is reported to be elevated in the failing ventricles. However, expression and transmural distribution of BNP in the atria remain unclear. Methods. Expression of ANP and BNP was immunohistochemically investigated in the right atrial (RA) specimens from 21 patients who had undergone cardiac surgery. The mRNA of specimens were quantitatively measured by Northern blot analysis and also evaluated by in situ hybridization. In addition, plasma levels of ANP and BNP were measured in the patients. Results. The BNP immunoreactivity was diffusely seen in RA tissue of patients with mean RA pressure (mRAP) of 5 mm Hg or more, but it was noted only in the subendocardial half of the atria of those with mRAP less than 5 mm Hg. There was a significant correlation between the incidence of BNP-positive myocytes and mRAP (r = 0.850, p < 0.0001). Conversely, ANP-positive myocytes were found diffusely in all cases. In Northern blot analysis, the mRNAs levels of ANP and BNP in the atrial tissue were positively correlated with the mRAP (ANP, p = 0.775, p < 0.005 and BNP, p = 0.771, p < 0.005). In situ hybridization confirmed these findings. The mRNA levels were significantly correlated to each other (r = 0.845, p < 0.0002). Plasma ANP and BNP levels were elevated in the patients compared with that in controls; however, none were significantly correlated with the mRAP. Conclusions. Expression of BNP and BNP mRNA is augmented in the atria with increased pressure, and distributed predominantly in the subendocardial side. The level of BNP mRNA was well correlated with that of ANP mRNA. Thus, these two genes might be commonly regulated in response to atrial pressure.

UR - http://www.scopus.com/inward/record.url?scp=0032420487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032420487&partnerID=8YFLogxK

U2 - 10.1016/S0735-1097(98)00494-X

DO - 10.1016/S0735-1097(98)00494-X

M3 - Article

C2 - 9857859

AN - SCOPUS:0032420487

VL - 32

SP - 1832

EP - 1838

JO - Journal of the American College of Cardiology

JF - Journal of the American College of Cardiology

SN - 0735-1097

IS - 7

ER -