Expression and function of adhesion molecules on human hematopoietic stem cells

CD34+ LFA-1- cells are more primitive than CD34+ LFA-1+ cells

Yuji Gunji, Mitsuru Nakamura, Toshihiko Hagiwara, Kayoko Hayakawa, Hirohisa Matsushita, Hisao Osawa, Kazunari Nagayoshi, Hiromitsu Nakauchi, Masayoshi Yanagisawa, Yasusada Miura, Toshio Suda

Research output: Contribution to journalArticle

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Abstract

Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). With this, we analyzed the phenotypes of CD34+ HSCs and showed that primitive HSCs or CD34+CD33- cells expressed adhesion molecules such as CD43, CD44, CD11a, CD11c, CD18, and leukocyte adhesion molecule (LAM-1). The more primitive hematopoietic cells or CD34+CD38- cells also expressed CD11a and CD18 with an incidence of 20% to 30%. To clarify the role of adhesion molecules in HSCs, we examined the colony forming capacity after long-term culture with allogeneic irradiated stromal layers. Among CD34+CD33- cells, CD18+ cells gave rise to colony-forming cells (CFCs) on stromal layers, but reached a maximum at week 2, after which the number of generated CFCs decreased. On the other hand, CD18- cells generated less CFCs than CD18+ cells at 2 to 3 weeks, but increased after 4 weeks of culture. When CD18 or CD11a antibody was added to a coculture system of CD34+CD33- cells with stromal layers, the number of generated CFCs decreased significantly compared with the no antibody control. Leukocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) was expressed on some populations of hematopoietic cells and contributed to the proliferation by interacting with stromal cells. However, more primitive cells capable of reconstituting hematopoiesis did not express LFA-1. These data provide a rationale for the administration of anti-LFA-1 antibody after bone marrow transplantation for reducing the graft failure.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalBlood
Volume80
Issue number2
Publication statusPublished - 1992 Jul 15

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Lymphocyte Function-Associated Antigen-1
Hematopoietic Stem Cells
Stem cells
Adhesion
Molecules
Antibodies
Transplantation (surgical)
L-Selectin
Cell adhesion
Grafts
Stromal Cells
Bone
Fluorescence
Cells
Phenotype
Hematopoiesis
Cell Adhesion Molecules
Coculture Techniques
Bone Marrow Transplantation

ASJC Scopus subject areas

  • Hematology

Cite this

Gunji, Y., Nakamura, M., Hagiwara, T., Hayakawa, K., Matsushita, H., Osawa, H., ... Suda, T. (1992). Expression and function of adhesion molecules on human hematopoietic stem cells: CD34+ LFA-1- cells are more primitive than CD34+ LFA-1+ cells. Blood, 80(2), 429-436.

Expression and function of adhesion molecules on human hematopoietic stem cells : CD34+ LFA-1- cells are more primitive than CD34+ LFA-1+ cells. / Gunji, Yuji; Nakamura, Mitsuru; Hagiwara, Toshihiko; Hayakawa, Kayoko; Matsushita, Hirohisa; Osawa, Hisao; Nagayoshi, Kazunari; Nakauchi, Hiromitsu; Yanagisawa, Masayoshi; Miura, Yasusada; Suda, Toshio.

In: Blood, Vol. 80, No. 2, 15.07.1992, p. 429-436.

Research output: Contribution to journalArticle

Gunji, Y, Nakamura, M, Hagiwara, T, Hayakawa, K, Matsushita, H, Osawa, H, Nagayoshi, K, Nakauchi, H, Yanagisawa, M, Miura, Y & Suda, T 1992, 'Expression and function of adhesion molecules on human hematopoietic stem cells: CD34+ LFA-1- cells are more primitive than CD34+ LFA-1+ cells', Blood, vol. 80, no. 2, pp. 429-436.
Gunji Y, Nakamura M, Hagiwara T, Hayakawa K, Matsushita H, Osawa H et al. Expression and function of adhesion molecules on human hematopoietic stem cells: CD34+ LFA-1- cells are more primitive than CD34+ LFA-1+ cells. Blood. 1992 Jul 15;80(2):429-436.
Gunji, Yuji ; Nakamura, Mitsuru ; Hagiwara, Toshihiko ; Hayakawa, Kayoko ; Matsushita, Hirohisa ; Osawa, Hisao ; Nagayoshi, Kazunari ; Nakauchi, Hiromitsu ; Yanagisawa, Masayoshi ; Miura, Yasusada ; Suda, Toshio. / Expression and function of adhesion molecules on human hematopoietic stem cells : CD34+ LFA-1- cells are more primitive than CD34+ LFA-1+ cells. In: Blood. 1992 ; Vol. 80, No. 2. pp. 429-436.
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abstract = "Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). With this, we analyzed the phenotypes of CD34+ HSCs and showed that primitive HSCs or CD34+CD33- cells expressed adhesion molecules such as CD43, CD44, CD11a, CD11c, CD18, and leukocyte adhesion molecule (LAM-1). The more primitive hematopoietic cells or CD34+CD38- cells also expressed CD11a and CD18 with an incidence of 20{\%} to 30{\%}. To clarify the role of adhesion molecules in HSCs, we examined the colony forming capacity after long-term culture with allogeneic irradiated stromal layers. Among CD34+CD33- cells, CD18+ cells gave rise to colony-forming cells (CFCs) on stromal layers, but reached a maximum at week 2, after which the number of generated CFCs decreased. On the other hand, CD18- cells generated less CFCs than CD18+ cells at 2 to 3 weeks, but increased after 4 weeks of culture. When CD18 or CD11a antibody was added to a coculture system of CD34+CD33- cells with stromal layers, the number of generated CFCs decreased significantly compared with the no antibody control. Leukocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) was expressed on some populations of hematopoietic cells and contributed to the proliferation by interacting with stromal cells. However, more primitive cells capable of reconstituting hematopoiesis did not express LFA-1. These data provide a rationale for the administration of anti-LFA-1 antibody after bone marrow transplantation for reducing the graft failure.",
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AU - Nagayoshi, Kazunari

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AU - Miura, Yasusada

AU - Suda, Toshio

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