Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells

Michihiro Yano, Atsushi Iwama, Hitoshi Nishio, Junko Suda, Goro Takada, Toshio Suda

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Two highly related receptor tyrosine kinases, TIE and TEK, comprise a family of endothelial cell-specific kinase. We established monoclonal antibodies against them and performed detailed analyses on their expression and function in murine hematopoietic stem cells (HSCs). TIE and TEK were expressed on 23.7% and 33.3% of lineage marker-negative, c-Kit+ and Sca-1+ (Lin- c-Kit+ Sca-1+) HSCs that contain the majority of day-12 colony- forming units-spleen (CFU-S) and long-term reconstituting cells, but not committed progenitor cells, Lin- c-Kit+ Sca-1+ cells were further divided by the expression of TIE and TEK. TIE+ and TEK+ HSCs as well as each negative counterpart contained high proliferative potential colony-forming cells and differentiated into lymphoid and myeloid progenies both in vitro and in vivo. However, day-12 CFU-S were enriched in TIE+ and TEK+ HSCs. Our findings define TIE and TEK as novel stem cell marker antigens that segregate day-12 CFU-S, and provide evidence of novel signaling pathways that are involved in the functional regulation of HSCs at a specific stage of differentiation, particularly of day-12 CFU-S.

Original languageEnglish
Pages (from-to)4317-4326
Number of pages10
JournalBlood
Volume89
Issue number12
Publication statusPublished - 1997 Jun 15

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TIE Receptors
Hematopoietic Stem Cells
Stem cells
Stem Cells
Spleen
Differentiation Antigens
Endothelial cells
Phosphotransferases
Endothelial Cells
Monoclonal Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Yano, M., Iwama, A., Nishio, H., Suda, J., Takada, G., & Suda, T. (1997). Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells. Blood, 89(12), 4317-4326.

Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells. / Yano, Michihiro; Iwama, Atsushi; Nishio, Hitoshi; Suda, Junko; Takada, Goro; Suda, Toshio.

In: Blood, Vol. 89, No. 12, 15.06.1997, p. 4317-4326.

Research output: Contribution to journalArticle

Yano, M, Iwama, A, Nishio, H, Suda, J, Takada, G & Suda, T 1997, 'Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells', Blood, vol. 89, no. 12, pp. 4317-4326.
Yano M, Iwama A, Nishio H, Suda J, Takada G, Suda T. Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells. Blood. 1997 Jun 15;89(12):4317-4326.
Yano, Michihiro ; Iwama, Atsushi ; Nishio, Hitoshi ; Suda, Junko ; Takada, Goro ; Suda, Toshio. / Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells. In: Blood. 1997 ; Vol. 89, No. 12. pp. 4317-4326.
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