Expression and function of NJ-1 surface antigen in megakaryopoiesis

Hua Tang; Xiu Qin Zhang; Takeshi Naruse; Kazuyuki Ohbo; Toshio Suda

doi:10.1006/bbrc.2002.6721

Expression and function of NJ-1 surface antigen in megakaryopoiesis

Hua Tang, Xiu Qin Zhang, Takeshi Naruse, Kazuyuki Ohbo, Toshio Suda

The Sakaguchi Laboratory of Developmental Biology Department of Cell Differentiation

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1⁺MKs and NJ-1⁻MKs. NJ-1⁺MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1⁺MKs have a high adherent potential to endothelial cells comparing with NJ-1⁻MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1⁺MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.

Original language	English
Pages (from-to)	667-674
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	292
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.2002.6721
Publication status	Published - 2002 Jan 1

Keywords

Cell adhesion
Endothelial cells
Megakaryopoiesis
NJ-1
Proplatelet formation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Expression and function of NJ-1 surface antigen in megakaryopoiesis",

abstract = "Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1+MKs and NJ-1−MKs. NJ-1+MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1+MKs have a high adherent potential to endothelial cells comparing with NJ-1−MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1+MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.",

keywords = "Cell adhesion, Endothelial cells, Megakaryopoiesis, NJ-1, Proplatelet formation",

author = "Hua Tang and Zhang, {Xiu Qin} and Takeshi Naruse and Kazuyuki Ohbo and Toshio Suda",

year = "2002",

month = jan,

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doi = "10.1006/bbrc.2002.6721",

language = "English",

volume = "292",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Expression and function of NJ-1 surface antigen in megakaryopoiesis

AU - Tang, Hua

AU - Zhang, Xiu Qin

AU - Naruse, Takeshi

AU - Ohbo, Kazuyuki

AU - Suda, Toshio

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1+MKs and NJ-1−MKs. NJ-1+MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1+MKs have a high adherent potential to endothelial cells comparing with NJ-1−MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1+MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.

AB - Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1+MKs and NJ-1−MKs. NJ-1+MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1+MKs have a high adherent potential to endothelial cells comparing with NJ-1−MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1+MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.

KW - Cell adhesion

KW - Endothelial cells

KW - Megakaryopoiesis

KW - NJ-1

KW - Proplatelet formation

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DO - 10.1006/bbrc.2002.6721

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Expression and function of NJ-1 surface antigen in megakaryopoiesis

Abstract

Keywords

ASJC Scopus subject areas

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