Expression and function of NJ-1 surface antigen in megakaryopoiesis

Hua Tang, Xiu Qin Zhang, Takeshi Naruse, Kazuyuki Ohbo, Toshio Suda

Research output: Contribution to journalArticle

Abstract

Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1+MKs and NJ-1-MKs. NJ-1+MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1+MKs have a high adherent potential to endothelial cells comparing with NJ-1-MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1+MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume292
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Megakaryocytes
Endothelial cells
Surface Antigens
Fusion reactions
Adhesion
Monoclonal Antibodies
Molecules
Proteins
Endothelial Cells
Stem Cells

Keywords

  • Cell adhesion
  • Endothelial cells
  • Megakaryopoiesis
  • NJ-1
  • Proplatelet formation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression and function of NJ-1 surface antigen in megakaryopoiesis. / Tang, Hua; Zhang, Xiu Qin; Naruse, Takeshi; Ohbo, Kazuyuki; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 292, No. 3, 2002, p. 667-674.

Research output: Contribution to journalArticle

Tang, Hua ; Zhang, Xiu Qin ; Naruse, Takeshi ; Ohbo, Kazuyuki ; Suda, Toshio. / Expression and function of NJ-1 surface antigen in megakaryopoiesis. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 292, No. 3. pp. 667-674.
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