Expression and function of NJ-1 surface antigen in megakaryopoiesis

Hua Tang, Xiu Qin Zhang, Takeshi Naruse, Kazuyuki Ohbo, Toshio Suda

Research output: Contribution to journalArticle

Abstract

Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1+MKs and NJ-1MKs. NJ-1+MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1+MKs have a high adherent potential to endothelial cells comparing with NJ-1MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1+MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume292
Issue number3
DOIs
Publication statusPublished - 2002 Jan 1

Keywords

  • Cell adhesion
  • Endothelial cells
  • Megakaryopoiesis
  • NJ-1
  • Proplatelet formation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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