Expression and function of the cholinergic system in immune cells

Takeshi Fujii, Masato Mashimo, Yasuhiro Moriwaki, Hidemi Misawa, Shiro Ono, Kazuhide Horiguchi, Koichiro Kawashima

Research output: Contribution to journalReview article

37 Citations (Scopus)

Abstract

T and B cells express most cholinergic system components-e.g., acetylcholine (ACh), choline acetyltransferase (ChAT), acetylcholinesterase, and both muscarinic and nicotinic ACh receptors (mAChRs and nAChRs, respectively). Using ChATBAC-eGFP transgenic mice, ChAT expression has been confirmed in T and B cells, dendritic cells, and macrophages. Moreover, T cell activation via T-cell receptor/CD3-mediated pathways upregulates ChAT mRNA expression and ACh synthesis, suggesting that this lymphocytic cholinergic system contributes to the regulation of immune function. Immune cells express all five mAChRs (M1-M5). Combined M1/M5 mAChR-deficient (M1/M5-KO) mice produce less antigen-specific antibody than wild-type (WT) mice. Furthermore, spleen cells in M1/M5-KO mice produce less tumor necrosis factor (TNF)-a and interleukin (IL)-6, suggesting M1/M5 mAChRs are involved in regulating pro-inflammatory cytokine and antibody production. Immune cells also frequently express the α2, α5, α6, α7, α9, and α10 nAChR subunits. α7 nAChR-deficient (α7-KO) mice produce more antigen-specific antibody than WT mice, and spleen cells from α7-KO mice produce more TNF-α and IL-6 than WT cells. This suggests that α7 nAChRs are involved in regulating cytokine production and thus modulate antibody production. Evidence also indicates that nicotine modulates immune responses by altering cytokine production and that α7 nAChR signaling contributes to immunomodulation through modification of T cell differentiation. Together, these findings suggest the involvement of both mAChRs and nAChRs in the regulation of immune function. The observation that vagus nerve stimulation protects mice from lethal endotoxin shock led to the notion of a cholinergic anti-inflammatory reflex pathway, and the spleen is an essential component of this anti-inflammatory reflex. Because the spleen lacks direct vagus innervation, it has been postulated that ACh synthesized by a subset of CD4+ T cells relays vagal nerve signals to α7 nAChRs on splenic macrophages, which downregulates TNF-α synthesis and release, thereby modulating inflammatory responses. However, because the spleen is innervated solely by the noradrenergic splenic nerve, confirmation of an anti-inflammatory reflex pathway involving the spleen requires several more hypotheses to be addressed. We will review and discuss these issues in the context of the cholinergic system in immune cells.

Original languageEnglish
Article number1085
JournalFrontiers in Immunology
Volume8
Issue numberSEP
DOIs
Publication statusPublished - 2017 Sep 6

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Cholinergic Agents
Immune System
Spleen
Choline O-Acetyltransferase
Acetylcholine
T-Lymphocytes
Reflex
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Cytokines
Antibody Formation
Interleukin-6
B-Lymphocytes
Macrophages
Vagus Nerve Stimulation
Antigens
Immunomodulation
Antibodies
Nicotinic Receptors
T-Lymphocyte Subsets

Keywords

  • Dendritic cell
  • Lymphocyte
  • mAChR
  • Macrophage
  • nAChR
  • SLURP-1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Expression and function of the cholinergic system in immune cells. / Fujii, Takeshi; Mashimo, Masato; Moriwaki, Yasuhiro; Misawa, Hidemi; Ono, Shiro; Horiguchi, Kazuhide; Kawashima, Koichiro.

In: Frontiers in Immunology, Vol. 8, No. SEP, 1085, 06.09.2017.

Research output: Contribution to journalReview article

Fujii, Takeshi ; Mashimo, Masato ; Moriwaki, Yasuhiro ; Misawa, Hidemi ; Ono, Shiro ; Horiguchi, Kazuhide ; Kawashima, Koichiro. / Expression and function of the cholinergic system in immune cells. In: Frontiers in Immunology. 2017 ; Vol. 8, No. SEP.
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