Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (114/A10), in the megakaryocytic lineage

Yun Duan, Takeshi Naruse, Mitsuru Nakamura, Yuji Yamaguchi, Toshiyuki Kawashima, Yoshihiro Morikawa, Toshio Kitamura, Toshio Suda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To analyze the functions of molecules expressed in hemopoietic progenitor cells, we obtained several monoclonal antibodies by immunizing Wistar rats with antigens from a murine immature leukemic cell line, DA-1. Here, we characterize one antibody designated NJ-1, which recognizes a 145-kd molecule, and identify the cDNA encoding the NJ-1 antigen by retrovirus-mediated expression cloning. Sequence analysis of the cDNA reveals that it is identical to a previously reported cDNA encoding a surface molecule of 573 amino acids recognized by monoclonal antibody 114/A10. Our studies show that expression of NJ-1 antigen is upregulated in a murine megakaryoblastic cell line, L8057, when it differentiates into a megakaryocytic lineage in response to 12-O-tetradecanoyl phorbol-13-acetate. Overexpression of NJ-1 antigen in L8057 cells inhibits cell adhesion to fibronectin, suggesting that it may act as a negative regulator of cell adhesion in the megakaryocytic lineage.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume253
Issue number2
DOIs
Publication statusPublished - 1998 Dec 18

Keywords

  • 114/A10
  • Cell adhesion
  • Hemopoietic progenitors
  • Megakaryocytes
  • NJ-1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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