Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (114/A10), in the megakaryocytic lineage

Yun Duan, Takeshi Naruse, Mitsuru Nakamura, Yuji Yamaguchi, Toshiyuki Kawashima, Yoshihiro Morikawa, Toshio Kitamura, Toshio Suda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To analyze the functions of molecules expressed in hemopoietic progenitor cells, we obtained several monoclonal antibodies by immunizing Wistar rats with antigens from a murine immature leukemic cell line, DA-1. Here, we characterize one antibody designated NJ-1, which recognizes a 145-kd molecule, and identify the cDNA encoding the NJ-1 antigen by retrovirus-mediated expression cloning. Sequence analysis of the cDNA reveals that it is identical to a previously reported cDNA encoding a surface molecule of 573 amino acids recognized by monoclonal antibody 114/A10. Our studies show that expression of NJ-1 antigen is upregulated in a murine megakaryoblastic cell line, L8057, when it differentiates into a megakaryocytic lineage in response to 12-O-tetradecanoyl phorbol-13-acetate. Overexpression of NJ-1 antigen in L8057 cells inhibits cell adhesion to fibronectin, suggesting that it may act as a negative regulator of cell adhesion in the megakaryocytic lineage.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume253
Issue number2
DOIs
Publication statusPublished - 1998 Dec 18

Fingerprint

Functional analysis
antineoplaston A10
Antigens
Complementary DNA
Cell adhesion
Cell Adhesion
Molecules
Monoclonal Antibodies
Cells
Cell Line
Cloning
Tetradecanoylphorbol Acetate
Retroviridae
Fibronectins
Sequence Analysis
Wistar Rats
Rats
Organism Cloning
Stem Cells
Amino Acids

Keywords

  • 114/A10
  • Cell adhesion
  • Hemopoietic progenitors
  • Megakaryocytes
  • NJ-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (114/A10), in the megakaryocytic lineage. / Duan, Yun; Naruse, Takeshi; Nakamura, Mitsuru; Yamaguchi, Yuji; Kawashima, Toshiyuki; Morikawa, Yoshihiro; Kitamura, Toshio; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 253, No. 2, 18.12.1998, p. 401-406.

Research output: Contribution to journalArticle

Duan, Y, Naruse, T, Nakamura, M, Yamaguchi, Y, Kawashima, T, Morikawa, Y, Kitamura, T & Suda, T 1998, 'Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (114/A10), in the megakaryocytic lineage', Biochemical and Biophysical Research Communications, vol. 253, no. 2, pp. 401-406. https://doi.org/10.1006/bbrc.1998.9700
Duan, Yun ; Naruse, Takeshi ; Nakamura, Mitsuru ; Yamaguchi, Yuji ; Kawashima, Toshiyuki ; Morikawa, Yoshihiro ; Kitamura, Toshio ; Suda, Toshio. / Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (114/A10), in the megakaryocytic lineage. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 253, No. 2. pp. 401-406.
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