Expression and proliferation-promoting role of diversin in the neuronally committed precursor cells migrating in the adult mouse brain

Makiko Ikeda, Yuki Hirota, Masanori Sakaguchi, Osamu Yamada, Yasuyuki S. Kida, Toshihiko Ogura, Takanobu Otsuka, Hideyuki Okano, Kazunobu Sawamoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus-mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain.

Original languageEnglish
Pages (from-to)2017-2026
Number of pages10
JournalStem Cells
Volume28
Issue number11
DOIs
Publication statusPublished - 2010 Nov

Fingerprint

Lateral Ventricles
Olfactory Bulb
Brain
Wnt Signaling Pathway
Retroviridae
Rodentia
Hippocampus
Cell Cycle
Neurons
diversin

Keywords

  • Diversin
  • Neurogenesis
  • Proliferation
  • Subventricular zone
  • Wnt signaling

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Expression and proliferation-promoting role of diversin in the neuronally committed precursor cells migrating in the adult mouse brain. / Ikeda, Makiko; Hirota, Yuki; Sakaguchi, Masanori; Yamada, Osamu; Kida, Yasuyuki S.; Ogura, Toshihiko; Otsuka, Takanobu; Okano, Hideyuki; Sawamoto, Kazunobu.

In: Stem Cells, Vol. 28, No. 11, 11.2010, p. 2017-2026.

Research output: Contribution to journalArticle

Ikeda, Makiko ; Hirota, Yuki ; Sakaguchi, Masanori ; Yamada, Osamu ; Kida, Yasuyuki S. ; Ogura, Toshihiko ; Otsuka, Takanobu ; Okano, Hideyuki ; Sawamoto, Kazunobu. / Expression and proliferation-promoting role of diversin in the neuronally committed precursor cells migrating in the adult mouse brain. In: Stem Cells. 2010 ; Vol. 28, No. 11. pp. 2017-2026.
@article{cd0a8c9ebd21452aa59eaae364f1b2ef,
title = "Expression and proliferation-promoting role of diversin in the neuronally committed precursor cells migrating in the adult mouse brain",
abstract = "The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus-mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain.",
keywords = "Diversin, Neurogenesis, Proliferation, Subventricular zone, Wnt signaling",
author = "Makiko Ikeda and Yuki Hirota and Masanori Sakaguchi and Osamu Yamada and Kida, {Yasuyuki S.} and Toshihiko Ogura and Takanobu Otsuka and Hideyuki Okano and Kazunobu Sawamoto",
year = "2010",
month = "11",
doi = "10.1002/stem.516",
language = "English",
volume = "28",
pages = "2017--2026",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Expression and proliferation-promoting role of diversin in the neuronally committed precursor cells migrating in the adult mouse brain

AU - Ikeda, Makiko

AU - Hirota, Yuki

AU - Sakaguchi, Masanori

AU - Yamada, Osamu

AU - Kida, Yasuyuki S.

AU - Ogura, Toshihiko

AU - Otsuka, Takanobu

AU - Okano, Hideyuki

AU - Sawamoto, Kazunobu

PY - 2010/11

Y1 - 2010/11

N2 - The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus-mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain.

AB - The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus-mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain.

KW - Diversin

KW - Neurogenesis

KW - Proliferation

KW - Subventricular zone

KW - Wnt signaling

UR - http://www.scopus.com/inward/record.url?scp=78649840469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649840469&partnerID=8YFLogxK

U2 - 10.1002/stem.516

DO - 10.1002/stem.516

M3 - Article

C2 - 20827749

AN - SCOPUS:78649840469

VL - 28

SP - 2017

EP - 2026

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 11

ER -