Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells

Yurie Yamamoto, Tetsuo Maruyama, Nozomi Sakai, Rei Sakurai, Aki Shimizu, Toshio Hamatani, Hirotaka Masuda, Hiroshi Uchida, Hisataka Sabe, Yasunori Yoshimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Decidual growth factors and locally produced cytokines are thought to activate specific phosphorylation signalling pathway(s), thereby eliciting a variety of decidual functions. We have previously reported the activation of c-Src tyrosine kinase during ovarian steroid-induced decidualization of cultured human endometrial stromal cells. As chicken c-Src is known to be activated upon dephosphorylation of tyrosine 527 (Y527, corresponding to Y530 in human), we here employed a monoclonal antibody, clone 28, directed against the active form of human c-Src whose Y530 is dephosphorylated, and investigated whether c-Src became dephosphorylated at Y530 and thereby activated during decidualization. We found that the active form of c-Src was up-regulated and demonstrated increased kinase activity during in-vitro decidualization. Immunohistochemistry revealed that decidual cells in early pregnancy decidua were intensely stained with clone 28 when compared with the stromal cells in the non-pregnant endometrium. Moreover, the active form of c-Src translocated from a perinuclear region to the cytoplasm upon decidualization. Thus, the Y530 dephosphorylation, kinase activation, and subcellular translocation of c-Src may be intracellular signalling events associated with decidualization in vivo as well as in vitro.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalMolecular Human Reproduction
Volume8
Issue number12
DOIs
Publication statusPublished - 2002 Dec 1

Fingerprint

Stromal Cells
Phosphotransferases
Clone Cells
Decidua
Endometrium
Tyrosine
Chickens
Intercellular Signaling Peptides and Proteins
Cytoplasm
Immunohistochemistry
Steroids
Monoclonal Antibodies
Phosphorylation
Cytokines
Pregnancy
CSK tyrosine-protein kinase
In Vitro Techniques

Keywords

  • c-Src
  • Decidualization
  • Progesterone
  • Tyrosine kinase
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells. / Yamamoto, Yurie; Maruyama, Tetsuo; Sakai, Nozomi; Sakurai, Rei; Shimizu, Aki; Hamatani, Toshio; Masuda, Hirotaka; Uchida, Hiroshi; Sabe, Hisataka; Yoshimura, Yasunori.

In: Molecular Human Reproduction, Vol. 8, No. 12, 01.12.2002, p. 1117-1124.

Research output: Contribution to journalArticle

Yamamoto, Yurie ; Maruyama, Tetsuo ; Sakai, Nozomi ; Sakurai, Rei ; Shimizu, Aki ; Hamatani, Toshio ; Masuda, Hirotaka ; Uchida, Hiroshi ; Sabe, Hisataka ; Yoshimura, Yasunori. / Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells. In: Molecular Human Reproduction. 2002 ; Vol. 8, No. 12. pp. 1117-1124.
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