Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells

Yurie Yamamoto, Tetsuo Maruyama, Nozomi Sakai, Rei Sakurai, Aki Shimizu, Toshio Hamatani, Hirotaka Masuda, Hiroshi Uchida, Hisataka Sabe, Yasunori Yoshimura

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15 Citations (Scopus)

Abstract

Decidual growth factors and locally produced cytokines are thought to activate specific phosphorylation signalling pathway(s), thereby eliciting a variety of decidual functions. We have previously reported the activation of c-Src tyrosine kinase during ovarian steroid-induced decidualization of cultured human endometrial stromal cells. As chicken c-Src is known to be activated upon dephosphorylation of tyrosine 527 (Y527, corresponding to Y530 in human), we here employed a monoclonal antibody, clone 28, directed against the active form of human c-Src whose Y530 is dephosphorylated, and investigated whether c-Src became dephosphorylated at Y530 and thereby activated during decidualization. We found that the active form of c-Src was up-regulated and demonstrated increased kinase activity during in-vitro decidualization. Immunohistochemistry revealed that decidual cells in early pregnancy decidua were intensely stained with clone 28 when compared with the stromal cells in the non-pregnant endometrium. Moreover, the active form of c-Src translocated from a perinuclear region to the cytoplasm upon decidualization. Thus, the Y530 dephosphorylation, kinase activation, and subcellular translocation of c-Src may be intracellular signalling events associated with decidualization in vivo as well as in vitro.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalMolecular Human Reproduction
Volume8
Issue number12
DOIs
Publication statusPublished - 2002 Dec 1

Keywords

  • Decidualization
  • Progesterone
  • Tyrosine kinase
  • Tyrosine phosphorylation
  • c-Src

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

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