Abstract
Aim: To examine the expression of αB-crystallin and vascular endothelial growth factor (VEGF) in conjunctival squamous cell carcinoma (CSCC). Materials and Methods: Seven CSCCs and three normal conjunctivas that were surgically excised were studied. Paraformaldehydefixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against αB-crystallin, its phosphorylated forms, and VEGF. In vitro experiments were conducted to investigate the effects of mitomycin C (MMC) treatment on the expression of αB-crystallin and VEGF secretion. Results: αB-Crystallin and VEGF were strongly expressed in CSCCs compared to normal conjunctivas. αB-Crystallin immunoreactivity was colocalized with that for VEGF in CSCCs, whereas these signals were reduced in CSCC tissues treated with MMC before excision. MMC treatment suppressed the αB-crystallin expression and VEGF secretion in cultured conjunctival cells in a dose-dependent manner. Conclusion: This study demonstrated αB-crystallin and VEGF expressions in human CSCCs, which may play a role in the pathogenesis. αBCrystallin expression, and VEGF secretion were reduced by MMC, indicating a novel therapeutic mechanism in MMC treatment for human CSCC.
| Original language | English |
|---|---|
| Pages (from-to) | 3745-3752 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 33 |
| Issue number | 9 |
| Publication status | Published - 2013 Sep |
| Externally published | Yes |
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Keywords
- αB-Crystallin
- Conjunctival squamous cell carcinoma
- Vascular endothelial growth factor
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma. / Dong, Zhenyu; Kase, Satoru; Ando, Ryo; Fukuhara, Junichi; Kinoshita, Satoshi; Dong, Yoko; Takashina, Saori; Kanda, Atsuhiro; Noda, Mika; Noda, Kousuke; Ishida, Susumu.
In: Anticancer Research, Vol. 33, No. 9, 09.2013, p. 3745-3752.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma
AU - Dong, Zhenyu
AU - Kase, Satoru
AU - Ando, Ryo
AU - Fukuhara, Junichi
AU - Kinoshita, Satoshi
AU - Dong, Yoko
AU - Takashina, Saori
AU - Kanda, Atsuhiro
AU - Noda, Mika
AU - Noda, Kousuke
AU - Ishida, Susumu
PY - 2013/9
Y1 - 2013/9
N2 - Aim: To examine the expression of αB-crystallin and vascular endothelial growth factor (VEGF) in conjunctival squamous cell carcinoma (CSCC). Materials and Methods: Seven CSCCs and three normal conjunctivas that were surgically excised were studied. Paraformaldehydefixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against αB-crystallin, its phosphorylated forms, and VEGF. In vitro experiments were conducted to investigate the effects of mitomycin C (MMC) treatment on the expression of αB-crystallin and VEGF secretion. Results: αB-Crystallin and VEGF were strongly expressed in CSCCs compared to normal conjunctivas. αB-Crystallin immunoreactivity was colocalized with that for VEGF in CSCCs, whereas these signals were reduced in CSCC tissues treated with MMC before excision. MMC treatment suppressed the αB-crystallin expression and VEGF secretion in cultured conjunctival cells in a dose-dependent manner. Conclusion: This study demonstrated αB-crystallin and VEGF expressions in human CSCCs, which may play a role in the pathogenesis. αBCrystallin expression, and VEGF secretion were reduced by MMC, indicating a novel therapeutic mechanism in MMC treatment for human CSCC.
AB - Aim: To examine the expression of αB-crystallin and vascular endothelial growth factor (VEGF) in conjunctival squamous cell carcinoma (CSCC). Materials and Methods: Seven CSCCs and three normal conjunctivas that were surgically excised were studied. Paraformaldehydefixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against αB-crystallin, its phosphorylated forms, and VEGF. In vitro experiments were conducted to investigate the effects of mitomycin C (MMC) treatment on the expression of αB-crystallin and VEGF secretion. Results: αB-Crystallin and VEGF were strongly expressed in CSCCs compared to normal conjunctivas. αB-Crystallin immunoreactivity was colocalized with that for VEGF in CSCCs, whereas these signals were reduced in CSCC tissues treated with MMC before excision. MMC treatment suppressed the αB-crystallin expression and VEGF secretion in cultured conjunctival cells in a dose-dependent manner. Conclusion: This study demonstrated αB-crystallin and VEGF expressions in human CSCCs, which may play a role in the pathogenesis. αBCrystallin expression, and VEGF secretion were reduced by MMC, indicating a novel therapeutic mechanism in MMC treatment for human CSCC.
KW - αB-Crystallin
KW - Conjunctival squamous cell carcinoma
KW - Vascular endothelial growth factor
UR - http://www.scopus.com/inward/record.url?scp=84885016020&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885016020&partnerID=8YFLogxK
M3 - Article
C2 - 24023305
AN - SCOPUS:84885016020
VL - 33
SP - 3745
EP - 3752
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 9
ER -