Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma

Zhenyu Dong, Satoru Kase, Ryo Ando, Junichi Fukuhara, Satoshi Kinoshita, Yoko Dong, Saori Takashina, Atsuhiro Kanda, Mika Noda, Kousuke Noda, Susumu Ishida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: To examine the expression of αB-crystallin and vascular endothelial growth factor (VEGF) in conjunctival squamous cell carcinoma (CSCC). Materials and Methods: Seven CSCCs and three normal conjunctivas that were surgically excised were studied. Paraformaldehydefixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against αB-crystallin, its phosphorylated forms, and VEGF. In vitro experiments were conducted to investigate the effects of mitomycin C (MMC) treatment on the expression of αB-crystallin and VEGF secretion. Results: αB-Crystallin and VEGF were strongly expressed in CSCCs compared to normal conjunctivas. αB-Crystallin immunoreactivity was colocalized with that for VEGF in CSCCs, whereas these signals were reduced in CSCC tissues treated with MMC before excision. MMC treatment suppressed the αB-crystallin expression and VEGF secretion in cultured conjunctival cells in a dose-dependent manner. Conclusion: This study demonstrated αB-crystallin and VEGF expressions in human CSCCs, which may play a role in the pathogenesis. αBCrystallin expression, and VEGF secretion were reduced by MMC, indicating a novel therapeutic mechanism in MMC treatment for human CSCC.

Original languageEnglish
Pages (from-to)3745-3752
Number of pages8
JournalAnticancer Research
Volume33
Issue number9
Publication statusPublished - 2013 Sep
Externally publishedYes

Fingerprint

Crystallins
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor B
Squamous Cell Carcinoma
Mitomycin
Conjunctiva
Therapeutics
Paraffin
Cultured Cells
Immunohistochemistry
Antibodies

Keywords

  • αB-Crystallin
  • Conjunctival squamous cell carcinoma
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dong, Z., Kase, S., Ando, R., Fukuhara, J., Kinoshita, S., Dong, Y., ... Ishida, S. (2013). Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma. Anticancer Research, 33(9), 3745-3752.

Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma. / Dong, Zhenyu; Kase, Satoru; Ando, Ryo; Fukuhara, Junichi; Kinoshita, Satoshi; Dong, Yoko; Takashina, Saori; Kanda, Atsuhiro; Noda, Mika; Noda, Kousuke; Ishida, Susumu.

In: Anticancer Research, Vol. 33, No. 9, 09.2013, p. 3745-3752.

Research output: Contribution to journalArticle

Dong, Z, Kase, S, Ando, R, Fukuhara, J, Kinoshita, S, Dong, Y, Takashina, S, Kanda, A, Noda, M, Noda, K & Ishida, S 2013, 'Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma', Anticancer Research, vol. 33, no. 9, pp. 3745-3752.
Dong Z, Kase S, Ando R, Fukuhara J, Kinoshita S, Dong Y et al. Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma. Anticancer Research. 2013 Sep;33(9):3745-3752.
Dong, Zhenyu ; Kase, Satoru ; Ando, Ryo ; Fukuhara, Junichi ; Kinoshita, Satoshi ; Dong, Yoko ; Takashina, Saori ; Kanda, Atsuhiro ; Noda, Mika ; Noda, Kousuke ; Ishida, Susumu. / Expression of αb-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma. In: Anticancer Research. 2013 ; Vol. 33, No. 9. pp. 3745-3752.
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AU - Kase, Satoru

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AU - Kinoshita, Satoshi

AU - Dong, Yoko

AU - Takashina, Saori

AU - Kanda, Atsuhiro

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AU - Noda, Kousuke

AU - Ishida, Susumu

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AB - Aim: To examine the expression of αB-crystallin and vascular endothelial growth factor (VEGF) in conjunctival squamous cell carcinoma (CSCC). Materials and Methods: Seven CSCCs and three normal conjunctivas that were surgically excised were studied. Paraformaldehydefixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against αB-crystallin, its phosphorylated forms, and VEGF. In vitro experiments were conducted to investigate the effects of mitomycin C (MMC) treatment on the expression of αB-crystallin and VEGF secretion. Results: αB-Crystallin and VEGF were strongly expressed in CSCCs compared to normal conjunctivas. αB-Crystallin immunoreactivity was colocalized with that for VEGF in CSCCs, whereas these signals were reduced in CSCC tissues treated with MMC before excision. MMC treatment suppressed the αB-crystallin expression and VEGF secretion in cultured conjunctival cells in a dose-dependent manner. Conclusion: This study demonstrated αB-crystallin and VEGF expressions in human CSCCs, which may play a role in the pathogenesis. αBCrystallin expression, and VEGF secretion were reduced by MMC, indicating a novel therapeutic mechanism in MMC treatment for human CSCC.

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