Abstract
Aim: To examine the expression of αB-crystallin and vascular endothelial growth factor (VEGF) in conjunctival squamous cell carcinoma (CSCC). Materials and Methods: Seven CSCCs and three normal conjunctivas that were surgically excised were studied. Paraformaldehydefixed, paraffin-embedded tissue sections were processed for immunohistochemistry with antibodies against αB-crystallin, its phosphorylated forms, and VEGF. In vitro experiments were conducted to investigate the effects of mitomycin C (MMC) treatment on the expression of αB-crystallin and VEGF secretion. Results: αB-Crystallin and VEGF were strongly expressed in CSCCs compared to normal conjunctivas. αB-Crystallin immunoreactivity was colocalized with that for VEGF in CSCCs, whereas these signals were reduced in CSCC tissues treated with MMC before excision. MMC treatment suppressed the αB-crystallin expression and VEGF secretion in cultured conjunctival cells in a dose-dependent manner. Conclusion: This study demonstrated αB-crystallin and VEGF expressions in human CSCCs, which may play a role in the pathogenesis. αBCrystallin expression, and VEGF secretion were reduced by MMC, indicating a novel therapeutic mechanism in MMC treatment for human CSCC.
| Original language | English |
|---|---|
| Pages (from-to) | 3745-3752 |
| Number of pages | 8 |
| Journal | Anticancer research |
| Volume | 33 |
| Issue number | 9 |
| Publication status | Published - 2013 Sep |
Keywords
- Conjunctival squamous cell carcinoma
- Vascular endothelial growth factor
- αB-Crystallin
ASJC Scopus subject areas
- Oncology
- Cancer Research