Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines

S. Sekiya, T. Nunoyama, H. Shirasawa, H. Kimura, M. Kawata, N. Iijima, Y. Sugimoto, T. Tsuruo, H. Takamizawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To investigate the possible role of the multidrug resistance phenotype to chemoresistance in human ovarian carcinoma, we have analyzed human multidrug resistance gene (mdr 1) expression in 8 human ovarian adenocarcinoma cell lines. An increase in P-glycoprotein level specific to multidrug-resistant tumor cells was not apparently associated with the increase in resistance to vincristine (VCR) or doxorubicin (Adriamycin). Mdr 1 transcripts (4.5 kilobases) were observed in the RNA preparation obtained from only one cell line (SHIN-3) that showed the highest resistance to both drugs in vitro and in vivo. No cell lines showed mdr 1 DNA amplification. These results suggest that the insensitivity of human ovarian carcinoma to chemotherapy could be partly explained by the expression of mdr 1.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalArchives of gynecology and obstetrics
Volume251
Issue number2
DOIs
Publication statusPublished - 1992 Jun

Keywords

  • Mdr 1 gene
  • Multidrug resistance
  • Ovarian carcinoma
  • P-glycoprotein

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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  • Cite this

    Sekiya, S., Nunoyama, T., Shirasawa, H., Kimura, H., Kawata, M., Iijima, N., Sugimoto, Y., Tsuruo, T., & Takamizawa, H. (1992). Expression of a human multidrug resistance gene in human ovarian carcinoma cell lines. Archives of gynecology and obstetrics, 251(2), 79-86. https://doi.org/10.1007/BF02759915