Expression of a provirus of human T cell leukaemia virus type I by DNA transfection

K. Mori, H. Sabe, Haruhiko Siomi, T. Iino, A. Tanaka, K. Takeuchi, K. Hirayoshi, M. Hatanaka

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We isolated the full length provirus of human T cell leukaemia virus type I (HTLV-I) from MT-2, a lymphoid cell line producing HTLV-I. In three non-lymphoid cell lines (COS7, human osteosarcoma HOS cells, and HeLa) this provirus expressed a transacting activity after co-transfection with a recombinant plasmid carrying a bacterial chloramphenicol acetyltransferase gene under the control of a long terminal repeat of HTLV-I provirus. The trans-acting protein p40 was detected by immunoprecipitation in transfected HOS cells. Structural proteins of HTLV-I, the gag and env products, were also formed and processed in the same manner as observed in MT-2 cells. In transfected HeLa cells, the p40 protein was mainly localized in the nucleus, while other structural proteins were detected in the cytoplasm and/or the membrane by indirect immunofluorescence. Syncytium formation was observed in HeLa cells after transfection. These results demonstrated that non-lymphoid cells could produce the major proteins of HTLV-I after DNA transfection of the cloned provirus.

Original languageEnglish
Pages (from-to)499-506
Number of pages8
JournalJournal of General Virology
Volume68
Issue number2
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Proviruses
Human T-lymphotropic virus 1
Transfection
HeLa Cells
DNA
Proteins
Cell Line
Chloramphenicol O-Acetyltransferase
Terminal Repeat Sequences
Osteosarcoma
Giant Cells
Indirect Fluorescent Antibody Technique
Immunoprecipitation
Cytoplasm
Plasmids
Lymphocytes
Membranes
Genes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Mori, K., Sabe, H., Siomi, H., Iino, T., Tanaka, A., Takeuchi, K., ... Hatanaka, M. (1987). Expression of a provirus of human T cell leukaemia virus type I by DNA transfection. Journal of General Virology, 68(2), 499-506.

Expression of a provirus of human T cell leukaemia virus type I by DNA transfection. / Mori, K.; Sabe, H.; Siomi, Haruhiko; Iino, T.; Tanaka, A.; Takeuchi, K.; Hirayoshi, K.; Hatanaka, M.

In: Journal of General Virology, Vol. 68, No. 2, 1987, p. 499-506.

Research output: Contribution to journalArticle

Mori, K, Sabe, H, Siomi, H, Iino, T, Tanaka, A, Takeuchi, K, Hirayoshi, K & Hatanaka, M 1987, 'Expression of a provirus of human T cell leukaemia virus type I by DNA transfection', Journal of General Virology, vol. 68, no. 2, pp. 499-506.
Mori, K. ; Sabe, H. ; Siomi, Haruhiko ; Iino, T. ; Tanaka, A. ; Takeuchi, K. ; Hirayoshi, K. ; Hatanaka, M. / Expression of a provirus of human T cell leukaemia virus type I by DNA transfection. In: Journal of General Virology. 1987 ; Vol. 68, No. 2. pp. 499-506.
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AU - Hirayoshi, K.

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