Expression of ADAMTS-4 (aggrecanase-1) and possible involvement in regression of lumbar disc herniation

Eiju Hatano, Takuya Fujita, Yoshimichi Ueda, Tetsuhito Okuda, Shogo Katsuda, Yasunori Okada, Tadami Matsumoto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

STUDY DESIGN. Examination of ADAMTS-4 expression, and cellular lineages, distribution, and numbers of ADAMTS-4 (aggrecanase-1) expressing cells in herniated lumbar intervertebral discs. OBJECTIVE. To determine the expression of ADAMTS-4, a metalloproteinase capable of digesting aggrecan, and its role in herniated lumbar intervertebral disc degradation. SUMMARY OF BACKGROUND DATA. Matrix metalloproteinases degrade extracellular matrix of herniated discs, but the mechanism of aggrecan degradation, the major component of intervertebral discs, is poorly understood. METHODS. Surgically resected herniated lumbar intervertebral discs from 22 patients were subclassified into protrusion, subligamentous extrusion, transligamentous extrusion, and sequestration types. Reverse transcriptase polymerase chain reaction, Western blot, and immunohistochemistry were used to evaluate ADAMTS-4 messenger ribonucleic acid and protein expression. RESULTS. Expression of ADAMTS-4 messenger ribonucleic acid and protein was shown in the samples of herniated lumbar intervertebral discs. Immunohistochemical staining showed that ADAMTS-4 was mainly localized in CD68-positive mononuclear cells in granulation and adjacent disc tissues. ADAMTS-4 positive cell counts were significantly higher in transligamentous extrusion and sequestration than protrusion and subligamentous extrusion types. Alcian blue staining showed a decrease of proteoglycan in transligamentous extrusion and sequestration cases. CONCLUSIONS. Macrophages infiltrating granulation and adjacent disc tissues express ADAMTS-4, suggesting its involvement in herniated disc regression.

Original languageEnglish
Pages (from-to)1426-1432
Number of pages7
JournalSpine
Volume31
Issue number13
DOIs
Publication statusPublished - 2006 Jun

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Intervertebral Disc
Aggrecans
Intervertebral Disc Displacement
RNA
Staining and Labeling
Intervertebral Disc Degeneration
Alcian Blue
Metalloproteases
Proteoglycans
Reverse Transcriptase Polymerase Chain Reaction
Matrix Metalloproteinases
Extracellular Matrix
Proteins
Cell Count
Western Blotting
Immunohistochemistry
Macrophages
ADAMTS4 Protein

Keywords

  • ADAMTS-4
  • Lumbar disc herniation
  • Spontaneous regression

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Orthopedics and Sports Medicine

Cite this

Hatano, E., Fujita, T., Ueda, Y., Okuda, T., Katsuda, S., Okada, Y., & Matsumoto, T. (2006). Expression of ADAMTS-4 (aggrecanase-1) and possible involvement in regression of lumbar disc herniation. Spine, 31(13), 1426-1432. https://doi.org/10.1097/01.brs.0000219954.67368.be

Expression of ADAMTS-4 (aggrecanase-1) and possible involvement in regression of lumbar disc herniation. / Hatano, Eiju; Fujita, Takuya; Ueda, Yoshimichi; Okuda, Tetsuhito; Katsuda, Shogo; Okada, Yasunori; Matsumoto, Tadami.

In: Spine, Vol. 31, No. 13, 06.2006, p. 1426-1432.

Research output: Contribution to journalArticle

Hatano, E, Fujita, T, Ueda, Y, Okuda, T, Katsuda, S, Okada, Y & Matsumoto, T 2006, 'Expression of ADAMTS-4 (aggrecanase-1) and possible involvement in regression of lumbar disc herniation', Spine, vol. 31, no. 13, pp. 1426-1432. https://doi.org/10.1097/01.brs.0000219954.67368.be
Hatano, Eiju ; Fujita, Takuya ; Ueda, Yoshimichi ; Okuda, Tetsuhito ; Katsuda, Shogo ; Okada, Yasunori ; Matsumoto, Tadami. / Expression of ADAMTS-4 (aggrecanase-1) and possible involvement in regression of lumbar disc herniation. In: Spine. 2006 ; Vol. 31, No. 13. pp. 1426-1432.
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AU - Katsuda, Shogo

AU - Okada, Yasunori

AU - Matsumoto, Tadami

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N2 - STUDY DESIGN. Examination of ADAMTS-4 expression, and cellular lineages, distribution, and numbers of ADAMTS-4 (aggrecanase-1) expressing cells in herniated lumbar intervertebral discs. OBJECTIVE. To determine the expression of ADAMTS-4, a metalloproteinase capable of digesting aggrecan, and its role in herniated lumbar intervertebral disc degradation. SUMMARY OF BACKGROUND DATA. Matrix metalloproteinases degrade extracellular matrix of herniated discs, but the mechanism of aggrecan degradation, the major component of intervertebral discs, is poorly understood. METHODS. Surgically resected herniated lumbar intervertebral discs from 22 patients were subclassified into protrusion, subligamentous extrusion, transligamentous extrusion, and sequestration types. Reverse transcriptase polymerase chain reaction, Western blot, and immunohistochemistry were used to evaluate ADAMTS-4 messenger ribonucleic acid and protein expression. RESULTS. Expression of ADAMTS-4 messenger ribonucleic acid and protein was shown in the samples of herniated lumbar intervertebral discs. Immunohistochemical staining showed that ADAMTS-4 was mainly localized in CD68-positive mononuclear cells in granulation and adjacent disc tissues. ADAMTS-4 positive cell counts were significantly higher in transligamentous extrusion and sequestration than protrusion and subligamentous extrusion types. Alcian blue staining showed a decrease of proteoglycan in transligamentous extrusion and sequestration cases. CONCLUSIONS. Macrophages infiltrating granulation and adjacent disc tissues express ADAMTS-4, suggesting its involvement in herniated disc regression.

AB - STUDY DESIGN. Examination of ADAMTS-4 expression, and cellular lineages, distribution, and numbers of ADAMTS-4 (aggrecanase-1) expressing cells in herniated lumbar intervertebral discs. OBJECTIVE. To determine the expression of ADAMTS-4, a metalloproteinase capable of digesting aggrecan, and its role in herniated lumbar intervertebral disc degradation. SUMMARY OF BACKGROUND DATA. Matrix metalloproteinases degrade extracellular matrix of herniated discs, but the mechanism of aggrecan degradation, the major component of intervertebral discs, is poorly understood. METHODS. Surgically resected herniated lumbar intervertebral discs from 22 patients were subclassified into protrusion, subligamentous extrusion, transligamentous extrusion, and sequestration types. Reverse transcriptase polymerase chain reaction, Western blot, and immunohistochemistry were used to evaluate ADAMTS-4 messenger ribonucleic acid and protein expression. RESULTS. Expression of ADAMTS-4 messenger ribonucleic acid and protein was shown in the samples of herniated lumbar intervertebral discs. Immunohistochemical staining showed that ADAMTS-4 was mainly localized in CD68-positive mononuclear cells in granulation and adjacent disc tissues. ADAMTS-4 positive cell counts were significantly higher in transligamentous extrusion and sequestration than protrusion and subligamentous extrusion types. Alcian blue staining showed a decrease of proteoglycan in transligamentous extrusion and sequestration cases. CONCLUSIONS. Macrophages infiltrating granulation and adjacent disc tissues express ADAMTS-4, suggesting its involvement in herniated disc regression.

KW - ADAMTS-4

KW - Lumbar disc herniation

KW - Spontaneous regression

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