Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage

Satoko Naito, Takayuki Shiomi, Aiko Okada, Tokuhiro Kimura, Miyuki Chijiiwa, Yoshinari Fujita, Taku Yatabe, Koichiro Komiya, Hiroyuki Enomoto, Kyosuke Fujikawa, Yasunori Okada

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)1, 4, 5, 8, 9 and 15, members of the ADAMTS gene family, have the ability to degrade a major cartilage proteoglycan, aggrecan, at the specific sites, and thus are called 'aggrecanases'. The expression of these ADAMTS species was examined in human osteoarthritic articular cartilage on reverse transcription-polymerase chain reaction. The results demonstrated the predominant expression of ADAMTS4 in osteoarthritic cartilage, while ADAMTS5 was constitutively expressed in osteoarthritic and normal cartilage. ADAMTS9 was expressed mainly in normal cartilage, whereas no or negligible expression of ADAMTS1, 8 and 15 was observed in either osteoarthritic or normal cartilage. In situ hybridization for ADAMTS4 indicated that chondrocytes in osteoarthritic cartilage expressed the mRNA. Two monoclonal antibodies to ADAMTS4 were developed, and immunolocalized ADAMTS4 to chondrocytes in the proteoglycan-depleted zones of osteoarthritic cartilage, showing a direct correlation with the Mankin scores. Immunoblotting indicated a major protein band of 58 kDa in the chondrocyte culture media and osteoarthritic cartilage tissue homogenates. These data demonstrate that among the six ADAMTS species, ADAMTS4 is mainly expressed in an active form in osteoarthritic cartilage, and suggest that ADAMTS4 may play an important role in the degradation of aggrecan in human osteoarthritic cartilage.

Original languageEnglish
Pages (from-to)703-711
Number of pages9
JournalPathology International
Volume57
Issue number11
DOIs
Publication statusPublished - 2007 Nov

Fingerprint

Cartilage
Disintegrins
Metalloproteases
Thrombospondins
Chondrocytes
Aggrecans
Proteoglycans
ADAMTS4 Protein
Thrombospondin 1
Articular Cartilage
Immunoblotting
Reverse Transcription
In Situ Hybridization
Culture Media
Monoclonal Antibodies
Polymerase Chain Reaction
Messenger RNA
Genes

Keywords

  • ADAMTS4
  • Aggrecanase
  • Cartilage destruction
  • Osteoarthritis
  • Proteoglycan degradation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Naito, S., Shiomi, T., Okada, A., Kimura, T., Chijiiwa, M., Fujita, Y., ... Okada, Y. (2007). Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage. Pathology International, 57(11), 703-711. https://doi.org/10.1111/j.1440-1827.2007.02167.x

Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage. / Naito, Satoko; Shiomi, Takayuki; Okada, Aiko; Kimura, Tokuhiro; Chijiiwa, Miyuki; Fujita, Yoshinari; Yatabe, Taku; Komiya, Koichiro; Enomoto, Hiroyuki; Fujikawa, Kyosuke; Okada, Yasunori.

In: Pathology International, Vol. 57, No. 11, 11.2007, p. 703-711.

Research output: Contribution to journalArticle

Naito, S, Shiomi, T, Okada, A, Kimura, T, Chijiiwa, M, Fujita, Y, Yatabe, T, Komiya, K, Enomoto, H, Fujikawa, K & Okada, Y 2007, 'Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage', Pathology International, vol. 57, no. 11, pp. 703-711. https://doi.org/10.1111/j.1440-1827.2007.02167.x
Naito S, Shiomi T, Okada A, Kimura T, Chijiiwa M, Fujita Y et al. Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage. Pathology International. 2007 Nov;57(11):703-711. https://doi.org/10.1111/j.1440-1827.2007.02167.x
Naito, Satoko ; Shiomi, Takayuki ; Okada, Aiko ; Kimura, Tokuhiro ; Chijiiwa, Miyuki ; Fujita, Yoshinari ; Yatabe, Taku ; Komiya, Koichiro ; Enomoto, Hiroyuki ; Fujikawa, Kyosuke ; Okada, Yasunori. / Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage. In: Pathology International. 2007 ; Vol. 57, No. 11. pp. 703-711.
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