Expression of AIRE gene in peripheral monocyte/dendritic cell lineage

Kazuhiko Kogawa, Seiho Nagafuchi, Hitoshi Katsuta, Jun Kudoh, Sadafumi Tamiya, Yumiko Sakai, Nobuyoshi Shimizu, Mine Harada

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102 Citations (Scopus)

Abstract

The responsible gene for autoimmune polyglandular syndrome type 1, known as autoimmune regulator (AIRE), was identified by positional cloning. The AIRE gene was reported to be expressed in the thymus medulla and lymph nodes. However, an expression of the AIRE gene in peripheral blood cells has not yet been reported. In the present study, we found that the AIRE gene was restrictively expressed in peripheral CD14-positive monocytes but not in CD4-positive T cells nor polymorphonuclear cells, as assessed by RT-PCR. Moreover, immunocytochemical study revealed the expression of the AIRE protein not only in CD14-positive monocytes but also in differentiated dendritic cells, cultured in RPMI1640 medium containing 800 U/ml GM-CSF, 1000 U/ml IL-4 and 100 U/ml TNF-α. Thus, it was concluded that the AIRE gene is restrictively expressed in the peripheral monocyte/dendritic cell lineage.

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalImmunology Letters
Volume80
Issue number3
DOIs
Publication statusPublished - 2002 Mar 1

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Keywords

  • Autoimmune polyglandular syndrome type 1
  • Autoimmune regulator gene
  • Autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy
  • Dendritic cells
  • Monocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Kogawa, K., Nagafuchi, S., Katsuta, H., Kudoh, J., Tamiya, S., Sakai, Y., Shimizu, N., & Harada, M. (2002). Expression of AIRE gene in peripheral monocyte/dendritic cell lineage. Immunology Letters, 80(3), 195-198. https://doi.org/10.1016/S0165-2478(01)00314-5