Expression of an isoform of the testis-specific estrogen sulfotransferase in the murine placenta during the late gestational period

K. Takehara, K. Kubushiro, Y. Iwamori, K. Tsukazaki, S. Nozawa, M. Iwamori

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3 Citations (Scopus)

Abstract

Cytosolic sulfotransferases play essential roles in regulating the activities and transfer of steroids. To evaluate their biological significance in the murine uterus and placenta during the course of gestation, we determined their activities with several steroids as substrates. Activated estrogen sulfotransferase (EST) was found in the placenta and uterus during the late gestational period. Reverse-transcribed cDNA of murine placental EST (mpEST) was isolated from mouse placenta at 18 days of gestation and its expression in the tissue coincided with a change in its enzyme activity. The open-reading frame of mpEST encodes a protein composed of 296 amino acids with a predicted molecular mass of 35.5 kDa and was revealed to be an isoform of the murine testis-specific EST gene (99.7%). Also, the amino acid sequence of mpEST showed 49.6 and 77.9% homology with human placental and endometrial EST, respectively, showing that it corresponds to human endometrial EST. COS-7 cells transfected with mpEST exhibited sulfotransferase activity with the phenolic hydroxy groups of steroids and artificial substrates. The best acceptor substrate was estrogen.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume394
Issue number2
DOIs
Publication statusPublished - 2001 Oct 15

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Keywords

  • Artificial substrate
  • COS-7 expression
  • Estrogen
  • Gestational period
  • Isozyme
  • Placenta
  • Steroid
  • Sulfotransferase
  • Uterus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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