Expression of Biologically Active Receptors for Natriuretic Peptides in the Human Uterus during Pregnancy

H. Itoh, N. Sagawa, M. Hasegawa, H. Nanno, F. Kobayashi, Y. Ihara, T. Mori, Y. Komatsu, S. I. Suga, T. Yoshimasa, H. Itoh, K. Nakao

Research output: Contribution to journalArticle

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Abstract

Human amnion cells secrete a large amount of brain natriuretic peptide (BNP). In the present study, to elucidate the possible roles of amniotic BNP during the course of human pregnancy, the tissue distributions of biologically active receptors of natriuretic peptides, atrial natriuretic peptide (ANP)-A receptor and ANP-B receptor in the human uterus during pregnancy were investigated by Northern blot analysis and an in vitro guanosine 3′,5′-cyclic phosphate (cGMP) generation assay, the second messenger of natriuretic peptides, using crude membrane preparations. Both ANP-A and ANP-B receptor mRNAs were detected in the tissues of decidua vera, chorion laeve, myometrium and placenta. The in vitro cGMP generation assay revealed that the expression of ANP-A receptor was more prominent than that of ANP-B receptor in these tissues. On the other hand, in amniotic tissue only ANP-B receptor mRNA was weakly expressed but ANP-A receptor mRNA was not detected.

Original languageEnglish
Pages (from-to)602-607
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number1
DOIs
Publication statusPublished - 1994 Aug 30
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor Receptors
Natriuretic Peptides
Atrial Natriuretic Factor
Uterus
Pregnancy
Tissue
Brain Natriuretic Peptide
Messenger RNA
Assays
Chorion
Decidua
Amnion
Myometrium
Guanosine
Second Messenger Systems
Tissue Distribution
Northern Blotting
Placenta
Phosphates
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Expression of Biologically Active Receptors for Natriuretic Peptides in the Human Uterus during Pregnancy. / Itoh, H.; Sagawa, N.; Hasegawa, M.; Nanno, H.; Kobayashi, F.; Ihara, Y.; Mori, T.; Komatsu, Y.; Suga, S. I.; Yoshimasa, T.; Itoh, H.; Nakao, K.

In: Biochemical and Biophysical Research Communications, Vol. 203, No. 1, 30.08.1994, p. 602-607.

Research output: Contribution to journalArticle

Itoh, H, Sagawa, N, Hasegawa, M, Nanno, H, Kobayashi, F, Ihara, Y, Mori, T, Komatsu, Y, Suga, SI, Yoshimasa, T, Itoh, H & Nakao, K 1994, 'Expression of Biologically Active Receptors for Natriuretic Peptides in the Human Uterus during Pregnancy', Biochemical and Biophysical Research Communications, vol. 203, no. 1, pp. 602-607. https://doi.org/10.1006/bbrc.1994.2225
Itoh, H. ; Sagawa, N. ; Hasegawa, M. ; Nanno, H. ; Kobayashi, F. ; Ihara, Y. ; Mori, T. ; Komatsu, Y. ; Suga, S. I. ; Yoshimasa, T. ; Itoh, H. ; Nakao, K. / Expression of Biologically Active Receptors for Natriuretic Peptides in the Human Uterus during Pregnancy. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 203, No. 1. pp. 602-607.
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