@article{5d1bf5d6b0a8401ba461e73ded758fbf,
title = "Expression of Brachyury-like T-box transcription factor Xbra3 in Xenopus embryo",
abstract = "The Xenopus Brachyury-like Xbra3 gene is a novel T-box gene that is closely associated with Xenopus Brachyury. The expression pattern of Xbra3 during development is similar to that of Xbra. During gastrulation Xbra3 is expressed in the marginal zone, with a gradient of increasing expression from ventral to dorsal. In the early neurula stage Xbra3 is expressed in the notochord and posterior mesoderm, but by the tailbud stage its expression is restricted to the forming tailbud and the posterior portion of the notochord.",
keywords = "Brachyury, Early mesoderm, Notochord tailbud, T-box gene",
author = "Tadayoshi Hayata and Akira Eisaki and Hiroki Kuroda and Makoto Asashima",
note = "Funding Information: Fig. 2A–H Comparison of the spatial patterns of expression of Xbra3 and Xbra by whole-mount in situ hybridization. A–C,G Xbra3 expression. D–F,H Xbra expression. A,D Vegetal views of expression of Xbra3 and Xbra at stage 10.5; top dorsal. B,E Vegetal views of expression of Xbra3 and Xbra at stage11; top dorsal; arrowheads expression of axial mesoderm. C,F Dorsal views of neurula embryos (stage 14). G,H Enlarged view of the tailbud of tailbud stage embryos (stage 31); arrow Xbra3 expression in the notochord. Whole mount in situ hybridization was performed as described previously (Harland 1991). Full-length Xbra3 antisense probe was prepared by digesting pXbra3 with BglII and transcribing with T7 RNA polymerase. Xbra3 sense probe was prepared by digesting pXbra3 with XhoI and transcribing with T3 RNA polymerase. Sense probe gave no background signals. Full-length antisense Xbra probe was prepared by digesting pXT1 (a gift from J. C. Smith) with EcoRV and transcribing with T7 RNA polymerase. We also used small probes containing only 3{\textquoteright} UTR of both genes. The same results was obtained, except that the UTR probes gave fainter signals (data not shown) Acknowledgements We are very grateful to Dr. Y. Etoh (Ajinomoto) for giving us the human recombinant activin A. This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan and by CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation.",
year = "1999",
doi = "10.1007/s004270050289",
language = "English",
volume = "209",
pages = "560--563",
journal = "Wilhelm Roux's Archives of Developmental Biology",
issn = "0949-944X",
publisher = "Springer Verlag",
number = "9",
}