Expression of cadherins and their undercoat proteins (α-, β-, and γ-catenins and p120) and accumulation of β-catenin with no gene mutations in synovial sarcoma

Haruhiro Sato, Tadashi Hasegawa, Yae Kanai, Yutaka Tsutsumi, Yoshiyuki Osamura, Yoshifumi Abe, Hideto Sakai, Setsuo Hirohashi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

E-cadherin, the major intercellular adhesion molecule of epithelial cells, is important in determining the architecture of sarcomas, especially those showing epithelioid features. In addition to its role in cell adhesion, β-catenin, a cadherin undercoat protein, has been shown to function as a downstream transcriptional activator of the Wnt/Wingless signaling pathway. In order to evaluate the significance of the cadherin cell adhesion system and the Wnt/Wingless signaling pathway in the morphogenesis and/or tumorigenesis of synovial sarcoma (a major type of sarcoma with epithelioid features), immunoreactivity for pan-cadherin, E-cadherin, and their undercoat proteins (α-, β-, and γ-catenins and p120) was evaluated in 15 synovial sarcomas. Immunoreactivity for pan-cadherin, E-cadherin, α-catenin, β-catenin, and p120 was observed in all 15 specimens. Immunoreactivity for pan-cadherin was stronger than that for E-cadherin. Expression of γ-catenin was detected in ten specimens. Although β-catenin was observed only at the cell-cell boundaries in four specimens, it was present in the nucleus and cytoplasm and at the cell-cell boundaries in the other 11, suggesting constitutional activation of the Wnt/Wingless signaling pathway in synovial sarcoma. Direct sequencing for exon 3 of the β-catenin gene, however, revealed no mutations in any of the 15 specimens. In conclusion, other types of cadherin besides E-cadherin, together with cadherin undercoat proteins, may play a role in cell adhesion in synovial sarcoma. Furthermore, mechanisms other than mutation of exon 3 of the β-catenin gene may activate the Wnt/Wingless signaling pathway in this type of tumor.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalVirchows Archiv
Volume438
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Synovial Sarcoma
Catenins
Cadherins
Mutation
Genes
Wnt Signaling Pathway
Cell Adhesion
Sarcoma
delta catenin
Exons
Cell Adhesion Molecules
Morphogenesis
Carcinogenesis
Cytoplasm
Proteins

Keywords

  • Cadherin
  • Catenin
  • p120
  • Synovial sarcoma
  • Wnt/Wingless signaling pathway

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of cadherins and their undercoat proteins (α-, β-, and γ-catenins and p120) and accumulation of β-catenin with no gene mutations in synovial sarcoma. / Sato, Haruhiro; Hasegawa, Tadashi; Kanai, Yae; Tsutsumi, Yutaka; Osamura, Yoshiyuki; Abe, Yoshifumi; Sakai, Hideto; Hirohashi, Setsuo.

In: Virchows Archiv, Vol. 438, No. 1, 2001, p. 23-30.

Research output: Contribution to journalArticle

Sato, Haruhiro ; Hasegawa, Tadashi ; Kanai, Yae ; Tsutsumi, Yutaka ; Osamura, Yoshiyuki ; Abe, Yoshifumi ; Sakai, Hideto ; Hirohashi, Setsuo. / Expression of cadherins and their undercoat proteins (α-, β-, and γ-catenins and p120) and accumulation of β-catenin with no gene mutations in synovial sarcoma. In: Virchows Archiv. 2001 ; Vol. 438, No. 1. pp. 23-30.
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AU - Hasegawa, Tadashi

AU - Kanai, Yae

AU - Tsutsumi, Yutaka

AU - Osamura, Yoshiyuki

AU - Abe, Yoshifumi

AU - Sakai, Hideto

AU - Hirohashi, Setsuo

PY - 2001

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AB - E-cadherin, the major intercellular adhesion molecule of epithelial cells, is important in determining the architecture of sarcomas, especially those showing epithelioid features. In addition to its role in cell adhesion, β-catenin, a cadherin undercoat protein, has been shown to function as a downstream transcriptional activator of the Wnt/Wingless signaling pathway. In order to evaluate the significance of the cadherin cell adhesion system and the Wnt/Wingless signaling pathway in the morphogenesis and/or tumorigenesis of synovial sarcoma (a major type of sarcoma with epithelioid features), immunoreactivity for pan-cadherin, E-cadherin, and their undercoat proteins (α-, β-, and γ-catenins and p120) was evaluated in 15 synovial sarcomas. Immunoreactivity for pan-cadherin, E-cadherin, α-catenin, β-catenin, and p120 was observed in all 15 specimens. Immunoreactivity for pan-cadherin was stronger than that for E-cadherin. Expression of γ-catenin was detected in ten specimens. Although β-catenin was observed only at the cell-cell boundaries in four specimens, it was present in the nucleus and cytoplasm and at the cell-cell boundaries in the other 11, suggesting constitutional activation of the Wnt/Wingless signaling pathway in synovial sarcoma. Direct sequencing for exon 3 of the β-catenin gene, however, revealed no mutations in any of the 15 specimens. In conclusion, other types of cadherin besides E-cadherin, together with cadherin undercoat proteins, may play a role in cell adhesion in synovial sarcoma. Furthermore, mechanisms other than mutation of exon 3 of the β-catenin gene may activate the Wnt/Wingless signaling pathway in this type of tumor.

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