Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma

Hiromi Kanno, Hiroshi Nishihara, Lei Wang, Sayaka Yuzawa, Hiroyuki Kobayashi, Masumi Tsuda, Taichi Kimura, Mishie Tanino, Shunsuke Terasaka, Shinya Tanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: CD163 is a 130-kDa transmembrane protein expressed in human monocytes and macrophages, and the aberrant expression of CD163 in breast and colorectal cancer associated with patients' poor prognosis was reported. Here, we analyzed the expression of CD163 in meningioma, a common intracranial tumor, and its molecular mechanism in association with meningioma progression. Methods: First, we performed immunohistochemical analysis using 50 human meningioma specimens. Next, we established CD163-overexpressing human meningioma cell lines and investigated its roles in tumor progression in vitro and in vivo. Results: Immunohistochemically, 26 of 50 human meningioma specimens (52.0%) were positive for CD163 in tumor cells, including benign grade I (48.5%) and grade II (71.4%) cases. Furthermore, CD163 expression was correlated with histological atypical parameters that directly predict the prognosis of meningioma. CD163-overexpressing meningioma cells showed significant suppression of apoptosis and accelerated tumor growth in nude mice. In addition, unexpected splenomegaly affiliated with the xenograft predicted tumor-derived granulocyte colony-stimulating factor (G-CSF) production, which was confirmed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Conclusions: To our knowledge, this is the first report that demonstrates CD163 expression in meningioma not only by immunohistochemistry but also by reverse-transcription polymerase chain reaction, using primary culture cells, and provides the novel molecular function of CD163 to prevent apoptosis through the production of G-CSF in meningioma.

Original languageEnglish
Pages (from-to)853-864
Number of pages12
JournalNeuro-oncology
Volume15
Issue number7
DOIs
Publication statusPublished - 2013 Jul 1
Externally publishedYes

Fingerprint

Meningioma
Granulocyte Colony-Stimulating Factor
Apoptosis
Neoplasms
Reverse Transcription
Polymerase Chain Reaction
Primary Cell Culture
Splenomegaly
Heterografts
Nude Mice
Monocytes
Colorectal Neoplasms
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Macrophages
Breast Neoplasms
Cell Line
Growth

Keywords

  • apoptosis
  • CD163
  • G-CSF
  • malignancy
  • meningioma

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma. / Kanno, Hiromi; Nishihara, Hiroshi; Wang, Lei; Yuzawa, Sayaka; Kobayashi, Hiroyuki; Tsuda, Masumi; Kimura, Taichi; Tanino, Mishie; Terasaka, Shunsuke; Tanaka, Shinya.

In: Neuro-oncology, Vol. 15, No. 7, 01.07.2013, p. 853-864.

Research output: Contribution to journalArticle

Kanno, H, Nishihara, H, Wang, L, Yuzawa, S, Kobayashi, H, Tsuda, M, Kimura, T, Tanino, M, Terasaka, S & Tanaka, S 2013, 'Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma', Neuro-oncology, vol. 15, no. 7, pp. 853-864. https://doi.org/10.1093/neuonc/not028
Kanno, Hiromi ; Nishihara, Hiroshi ; Wang, Lei ; Yuzawa, Sayaka ; Kobayashi, Hiroyuki ; Tsuda, Masumi ; Kimura, Taichi ; Tanino, Mishie ; Terasaka, Shunsuke ; Tanaka, Shinya. / Expression of CD163 prevents apoptosis through the production of granulocyte colony-stimulating factor in meningioma. In: Neuro-oncology. 2013 ; Vol. 15, No. 7. pp. 853-864.
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AU - Wang, Lei

AU - Yuzawa, Sayaka

AU - Kobayashi, Hiroyuki

AU - Tsuda, Masumi

AU - Kimura, Taichi

AU - Tanino, Mishie

AU - Terasaka, Shunsuke

AU - Tanaka, Shinya

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AB - Background: CD163 is a 130-kDa transmembrane protein expressed in human monocytes and macrophages, and the aberrant expression of CD163 in breast and colorectal cancer associated with patients' poor prognosis was reported. Here, we analyzed the expression of CD163 in meningioma, a common intracranial tumor, and its molecular mechanism in association with meningioma progression. Methods: First, we performed immunohistochemical analysis using 50 human meningioma specimens. Next, we established CD163-overexpressing human meningioma cell lines and investigated its roles in tumor progression in vitro and in vivo. Results: Immunohistochemically, 26 of 50 human meningioma specimens (52.0%) were positive for CD163 in tumor cells, including benign grade I (48.5%) and grade II (71.4%) cases. Furthermore, CD163 expression was correlated with histological atypical parameters that directly predict the prognosis of meningioma. CD163-overexpressing meningioma cells showed significant suppression of apoptosis and accelerated tumor growth in nude mice. In addition, unexpected splenomegaly affiliated with the xenograft predicted tumor-derived granulocyte colony-stimulating factor (G-CSF) production, which was confirmed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Conclusions: To our knowledge, this is the first report that demonstrates CD163 expression in meningioma not only by immunohistochemistry but also by reverse-transcription polymerase chain reaction, using primary culture cells, and provides the novel molecular function of CD163 to prevent apoptosis through the production of G-CSF in meningioma.

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