Expression of CD44R1 adhesion molecule in colon carcinomas and metastases

K. K. Tanabe, L. M. Ellis, Hideyuki Saya

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

CD44 is a cell surface adhesion molecule postulated to control lymphocyte recirculation by facilitating entry into lymphoid tissue. Tumour cells transfected to overexpress the epithelial variant CD44R1 readily gain access to lymph nodes and distant metastatic sites in animal models, possibly by mimicking circulating lymphocytes.. To investigate if human tumours display altered CD44 expression we performed reverse transcriptase a polymerase chain reaction (PCR) analysis of CD44 in 49 specimens from normal colonic mucosa, primary colon and rectal tumours, normal liver, and metastases of 20 patients. The haematopoietic variant CD44H was the principal isoform amplified in all of the specimens, However, 12/14 primary tumours and 16/16 metastatic tumours expressed CD44R1, compared with only 2 of 13 normal mucosa specimens. This increase in CD44R1 relative to CD44H expressed by human colon carcinoma cells may increase their metastatic potential. Moreover, the relative increase in PCR amplification of CD44R1 compared with that of CD44H may provide a sensitive method for detecting primary and metastatic colon carcinoma cells in small biopsy specimens.

Original languageEnglish
Pages (from-to)725-726
Number of pages2
JournalThe Lancet
Volume341
Issue number8847
DOIs
Publication statusPublished - 1993 Mar 20
Externally publishedYes

Fingerprint

Colon
Neoplasm Metastasis
Carcinoma
Neoplasms
Mucous Membrane
Lymphocytes
Small Cell Carcinoma
Cell Adhesion Molecules
Lymphoid Tissue
Rectal Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Protein Isoforms
Animal Models
Lymph Nodes
Biopsy
Polymerase Chain Reaction
Liver

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of CD44R1 adhesion molecule in colon carcinomas and metastases. / Tanabe, K. K.; Ellis, L. M.; Saya, Hideyuki.

In: The Lancet, Vol. 341, No. 8847, 20.03.1993, p. 725-726.

Research output: Contribution to journalArticle

Tanabe, K. K. ; Ellis, L. M. ; Saya, Hideyuki. / Expression of CD44R1 adhesion molecule in colon carcinomas and metastases. In: The Lancet. 1993 ; Vol. 341, No. 8847. pp. 725-726.
@article{5d914651b2da4414bd4e00ea3dd5486b,
title = "Expression of CD44R1 adhesion molecule in colon carcinomas and metastases",
abstract = "CD44 is a cell surface adhesion molecule postulated to control lymphocyte recirculation by facilitating entry into lymphoid tissue. Tumour cells transfected to overexpress the epithelial variant CD44R1 readily gain access to lymph nodes and distant metastatic sites in animal models, possibly by mimicking circulating lymphocytes.. To investigate if human tumours display altered CD44 expression we performed reverse transcriptase a polymerase chain reaction (PCR) analysis of CD44 in 49 specimens from normal colonic mucosa, primary colon and rectal tumours, normal liver, and metastases of 20 patients. The haematopoietic variant CD44H was the principal isoform amplified in all of the specimens, However, 12/14 primary tumours and 16/16 metastatic tumours expressed CD44R1, compared with only 2 of 13 normal mucosa specimens. This increase in CD44R1 relative to CD44H expressed by human colon carcinoma cells may increase their metastatic potential. Moreover, the relative increase in PCR amplification of CD44R1 compared with that of CD44H may provide a sensitive method for detecting primary and metastatic colon carcinoma cells in small biopsy specimens.",
author = "Tanabe, {K. K.} and Ellis, {L. M.} and Hideyuki Saya",
year = "1993",
month = "3",
day = "20",
doi = "10.1016/0140-6736(93)90490-8",
language = "English",
volume = "341",
pages = "725--726",
journal = "The Lancet",
issn = "0140-6736",
publisher = "Elsevier Limited",
number = "8847",

}

TY - JOUR

T1 - Expression of CD44R1 adhesion molecule in colon carcinomas and metastases

AU - Tanabe, K. K.

AU - Ellis, L. M.

AU - Saya, Hideyuki

PY - 1993/3/20

Y1 - 1993/3/20

N2 - CD44 is a cell surface adhesion molecule postulated to control lymphocyte recirculation by facilitating entry into lymphoid tissue. Tumour cells transfected to overexpress the epithelial variant CD44R1 readily gain access to lymph nodes and distant metastatic sites in animal models, possibly by mimicking circulating lymphocytes.. To investigate if human tumours display altered CD44 expression we performed reverse transcriptase a polymerase chain reaction (PCR) analysis of CD44 in 49 specimens from normal colonic mucosa, primary colon and rectal tumours, normal liver, and metastases of 20 patients. The haematopoietic variant CD44H was the principal isoform amplified in all of the specimens, However, 12/14 primary tumours and 16/16 metastatic tumours expressed CD44R1, compared with only 2 of 13 normal mucosa specimens. This increase in CD44R1 relative to CD44H expressed by human colon carcinoma cells may increase their metastatic potential. Moreover, the relative increase in PCR amplification of CD44R1 compared with that of CD44H may provide a sensitive method for detecting primary and metastatic colon carcinoma cells in small biopsy specimens.

AB - CD44 is a cell surface adhesion molecule postulated to control lymphocyte recirculation by facilitating entry into lymphoid tissue. Tumour cells transfected to overexpress the epithelial variant CD44R1 readily gain access to lymph nodes and distant metastatic sites in animal models, possibly by mimicking circulating lymphocytes.. To investigate if human tumours display altered CD44 expression we performed reverse transcriptase a polymerase chain reaction (PCR) analysis of CD44 in 49 specimens from normal colonic mucosa, primary colon and rectal tumours, normal liver, and metastases of 20 patients. The haematopoietic variant CD44H was the principal isoform amplified in all of the specimens, However, 12/14 primary tumours and 16/16 metastatic tumours expressed CD44R1, compared with only 2 of 13 normal mucosa specimens. This increase in CD44R1 relative to CD44H expressed by human colon carcinoma cells may increase their metastatic potential. Moreover, the relative increase in PCR amplification of CD44R1 compared with that of CD44H may provide a sensitive method for detecting primary and metastatic colon carcinoma cells in small biopsy specimens.

UR - http://www.scopus.com/inward/record.url?scp=0027499085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027499085&partnerID=8YFLogxK

U2 - 10.1016/0140-6736(93)90490-8

DO - 10.1016/0140-6736(93)90490-8

M3 - Article

C2 - 8095628

AN - SCOPUS:0027499085

VL - 341

SP - 725

EP - 726

JO - The Lancet

JF - The Lancet

SN - 0140-6736

IS - 8847

ER -