Expression of cell adhesion molecules in the salivary and lacrimal glands of Sjogren's syndrome

I. Saito, K. Terauchi, M. Shimuta, S. Nishiimura, K. Yoshino, Tsutomu Takeuchi, Kazuo Tsubota, N. Miyasaka

Research output: Contribution to journalArticle

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Abstract

We attempted to determine whether cell adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule- 1 (ICAM-1), and E-selectin (endothelial-leukocyte adhesion molecule-1; ELAM- 1), are involved in the lymphoid cell infiltration of the salivary and lacrimal glands in Sjogren's syndrome (SS) patients. Both immunohistochemical analysis and the reverse-transcripts polymerase chain reaction (RT-PCR) were used to analyze the expression of VCAM-1, ICAM-1, ELAM-1, very late antigen 4 (VLA-4 [α4, β1]), lymphocyte function-associated antigen-1 (LFA-1), interferon-γ(IFN-γ), tumor necrosis factor (TNF), and interleukin-1 β (IL- 1β). Immunohistochemical analysis of salivary gland biopsies from SS patients showed a marked expression of VCAM-1 and ICAM-1 in the venules surrounded by infiltrated CD4+ CD45RO+ T cells. E-selectin was expressed on vascular endothelium with weak intensity. Increased levels of VCAM-1, ICAM- 1, IFN-γ, and IL-1β mRNA were demonstrated by RT-PCR, whereas E-selectin mRNA were weakly expressed in SS lacrimal and salivary gland tissues. This is in contrast with strong expression of ELAM-1 in IL-1β-stimulated human umbilical vascular endothelial cells (HUVEC) in vitro. Cytokine-mediated up- regulation of VCAM-1 and ICAM-1 that facilitates the recruitment of VLA-4 and LFA-1 expressing T cells might contribute to lymphoid cell infiltration in the salivary and lacrimal glands in SS.

Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalJournal of Clinical Laboratory Analysis
Volume7
Issue number3
Publication statusPublished - 1993
Externally publishedYes

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Lacrimal Apparatus
E-Selectin
Sjogren's Syndrome
Cell Adhesion Molecules
Salivary Glands
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Integrin alpha4beta1
Interleukin-1
Lymphocyte Function-Associated Antigen-1
T-cells
Polymerase chain reaction
Infiltration
Lymphocytes
T-Lymphocytes
Umbilicus
Polymerase Chain Reaction
Messenger RNA
Venules
Biopsy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Saito, I., Terauchi, K., Shimuta, M., Nishiimura, S., Yoshino, K., Takeuchi, T., ... Miyasaka, N. (1993). Expression of cell adhesion molecules in the salivary and lacrimal glands of Sjogren's syndrome. Journal of Clinical Laboratory Analysis, 7(3), 180-187.

Expression of cell adhesion molecules in the salivary and lacrimal glands of Sjogren's syndrome. / Saito, I.; Terauchi, K.; Shimuta, M.; Nishiimura, S.; Yoshino, K.; Takeuchi, Tsutomu; Tsubota, Kazuo; Miyasaka, N.

In: Journal of Clinical Laboratory Analysis, Vol. 7, No. 3, 1993, p. 180-187.

Research output: Contribution to journalArticle

Saito, I, Terauchi, K, Shimuta, M, Nishiimura, S, Yoshino, K, Takeuchi, T, Tsubota, K & Miyasaka, N 1993, 'Expression of cell adhesion molecules in the salivary and lacrimal glands of Sjogren's syndrome', Journal of Clinical Laboratory Analysis, vol. 7, no. 3, pp. 180-187.
Saito, I. ; Terauchi, K. ; Shimuta, M. ; Nishiimura, S. ; Yoshino, K. ; Takeuchi, Tsutomu ; Tsubota, Kazuo ; Miyasaka, N. / Expression of cell adhesion molecules in the salivary and lacrimal glands of Sjogren's syndrome. In: Journal of Clinical Laboratory Analysis. 1993 ; Vol. 7, No. 3. pp. 180-187.
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AU - Saito, I.

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AU - Shimuta, M.

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AU - Yoshino, K.

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AU - Tsubota, Kazuo

AU - Miyasaka, N.

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N2 - We attempted to determine whether cell adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule- 1 (ICAM-1), and E-selectin (endothelial-leukocyte adhesion molecule-1; ELAM- 1), are involved in the lymphoid cell infiltration of the salivary and lacrimal glands in Sjogren's syndrome (SS) patients. Both immunohistochemical analysis and the reverse-transcripts polymerase chain reaction (RT-PCR) were used to analyze the expression of VCAM-1, ICAM-1, ELAM-1, very late antigen 4 (VLA-4 [α4, β1]), lymphocyte function-associated antigen-1 (LFA-1), interferon-γ(IFN-γ), tumor necrosis factor (TNF), and interleukin-1 β (IL- 1β). Immunohistochemical analysis of salivary gland biopsies from SS patients showed a marked expression of VCAM-1 and ICAM-1 in the venules surrounded by infiltrated CD4+ CD45RO+ T cells. E-selectin was expressed on vascular endothelium with weak intensity. Increased levels of VCAM-1, ICAM- 1, IFN-γ, and IL-1β mRNA were demonstrated by RT-PCR, whereas E-selectin mRNA were weakly expressed in SS lacrimal and salivary gland tissues. This is in contrast with strong expression of ELAM-1 in IL-1β-stimulated human umbilical vascular endothelial cells (HUVEC) in vitro. Cytokine-mediated up- regulation of VCAM-1 and ICAM-1 that facilitates the recruitment of VLA-4 and LFA-1 expressing T cells might contribute to lymphoid cell infiltration in the salivary and lacrimal glands in SS.

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