Expression of coxsackievirus and adenovirus receptor in hearts of rats with experimental autoimmune myocarditis

Masahiro Ito, Makoto Kodama, Masayoshi Masuko, Masayuki Yamaura, Koichi Fuse, Yumiko Uesugi, Satoru Hirono, Yuji Okura, Kiminori Kato, Yuko Hotta, Takao Honda, Ryozo Kuwano, Yoshifusa Aizawa

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)

Abstract

The expression of coxsackievirus and adenovirus receptor (CAR) was dominant in the brains and hearts of mice until the newborn phase. There is no detailed information concerning the relation between the expression of CAR and development of hearts. It is also uncertain whether CAR is able to be induced in adult hearts after cardiac injury. We demonstrated that CAR was abundant in the hearts of newborn rats but was barely detectable in the hearts of adult rats. The expression of CAR in rat hearts with experimental autoimmune myocarditis, which was induced by immunization of purified cardiac myosin, was serially investigated. Active myocarditis was observed from day 15 after immunization. By immunohistochemistry, cardiomyocytes were strongly stained for CAR antibody from days 24 to 42. CAR mRNA was also detected from days 18 to 30 by using reverse transcription-polymerase chain reaction. In the next experiment, the induction of CAR on isolated cardiomyocytes was investigated. CAR was barely detectable in cultured cardiomyocytes by Western blot analysis after isolation. This molecule gradually appeared along with the creation of clusters and beating of cardiomyocytes. Furthermore, the induction of CAR in cultured cardiomyocytes increased after supplement with conditioned medium of rat splenocytes activated by concanavalin A. In conclusion, rat CAR is expressed strongly in the hearts of newborn rats and is suppressed in those of adult rats. The expression of CAR is enhanced during the active phase of experimental autoimmune myocarditis and is induced by inflammatory mediators. CAR may play a role in cell-to-cell contact and adhesion of cardiomyocytes.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalCirculation research
Volume86
Issue number3
DOIs
Publication statusPublished - 2000 Feb 18
Externally publishedYes

Keywords

  • Cardiomyocytes
  • Coxsackievirus and adenovirus receptors
  • Myocarditis

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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