Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

Ryoji Fujimaki, Katsuhiko Hayashi, Naoko Watanabe, Taketo Yamada, Yoshiaki Toyama, Ken Ichi Tezuka, Nobumichi Hozumi

Research output: Contribution to journalArticle

4 Citations (Scopus)


Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

Original languageEnglish
Pages (from-to)270-273
Number of pages4
JournalJournal of Bone and Mineral Metabolism
Issue number3
Publication statusPublished - 2005 May 1



  • Chondrocyte
  • Cre-LoxP
  • α2(XI) collagen gene

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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