Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

Ryoji Fujimaki, Katsuhiko Hayashi, Naoko Watanabe, Taketo Yamada, Yoshiaki Toyama, Ken Ichi Tezuka, Nobumichi Hozumi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

Original languageEnglish
Pages (from-to)270-273
Number of pages4
JournalJournal of Bone and Mineral Metabolism
Volume23
Issue number3
DOIs
Publication statusPublished - 2005 May

Fingerprint

Chondrocytes
Collagen
Transgenic Mice
Genes
Forelimb
Gene Silencing
Osteogenesis
Cre recombinase

Keywords

  • α2(XI) collagen gene
  • Chondrocyte
  • Cre-LoxP

ASJC Scopus subject areas

  • Endocrinology

Cite this

Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter. / Fujimaki, Ryoji; Hayashi, Katsuhiko; Watanabe, Naoko; Yamada, Taketo; Toyama, Yoshiaki; Tezuka, Ken Ichi; Hozumi, Nobumichi.

In: Journal of Bone and Mineral Metabolism, Vol. 23, No. 3, 05.2005, p. 270-273.

Research output: Contribution to journalArticle

Fujimaki, Ryoji ; Hayashi, Katsuhiko ; Watanabe, Naoko ; Yamada, Taketo ; Toyama, Yoshiaki ; Tezuka, Ken Ichi ; Hozumi, Nobumichi. / Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter. In: Journal of Bone and Mineral Metabolism. 2005 ; Vol. 23, No. 3. pp. 270-273.
@article{c78f62ee38644bd98b92a05ffffd680b,
title = "Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter",
abstract = "Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.",
keywords = "α2(XI) collagen gene, Chondrocyte, Cre-LoxP",
author = "Ryoji Fujimaki and Katsuhiko Hayashi and Naoko Watanabe and Taketo Yamada and Yoshiaki Toyama and Tezuka, {Ken Ichi} and Nobumichi Hozumi",
year = "2005",
month = "5",
doi = "10.1007/s00774-004-0595-y",
language = "English",
volume = "23",
pages = "270--273",
journal = "Journal of Bone and Mineral Metabolism",
issn = "0914-8779",
publisher = "Springer Japan",
number = "3",

}

TY - JOUR

T1 - Expression of Cre recombinase in the mouse developing chondrocytes driven by the mouse α2(XI) collagen promoter

AU - Fujimaki, Ryoji

AU - Hayashi, Katsuhiko

AU - Watanabe, Naoko

AU - Yamada, Taketo

AU - Toyama, Yoshiaki

AU - Tezuka, Ken Ichi

AU - Hozumi, Nobumichi

PY - 2005/5

Y1 - 2005/5

N2 - Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

AB - Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse α2(XI) collagen gene. Crossing this mouse line with a reporter mouse revealed that Cre recombinase activity is observed specifically in developing chondrocytes. This chondrocyte-specific Cre expression was observed from the early stage of chondrocyte differentiation in forelimb at 12.5 dpc, but not expressed in mesenchymal condensations. This transgenic mouse line will be a suitable resource for the analysis of gene function in differentiating chondrocytes and the mechanism of endochondral ossification.

KW - α2(XI) collagen gene

KW - Chondrocyte

KW - Cre-LoxP

UR - http://www.scopus.com/inward/record.url?scp=18044381310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18044381310&partnerID=8YFLogxK

U2 - 10.1007/s00774-004-0595-y

DO - 10.1007/s00774-004-0595-y

M3 - Article

C2 - 15838632

AN - SCOPUS:18044381310

VL - 23

SP - 270

EP - 273

JO - Journal of Bone and Mineral Metabolism

JF - Journal of Bone and Mineral Metabolism

SN - 0914-8779

IS - 3

ER -