Abstract
The development of the rat adrenal gland during late gestational and neonatal stages was studied by following the expression of aldosterone synthase cytochrome P450 (P450aldo) and glucocorticoid-synthesizing cytochrome P450 (P45011β). Cells expressing P450aldo, a functional marker for the mineralocorticoid-synthesizing zona glomerulosa, were not detected until day 20 of fetal age, i.e., 2 days before birth, although the zona glomerulosa cells were histologically recognizable at the 18th day of gestation. The intensity of P450aldo staining thereafter became stronger with age in the outer portion of the cortex. Cells expressing P45011β, a marker for the glucocorticoid-producing zona fasciculata, were present in the fetal adrenals on the 18th day. P45011β-positive cells were distributed over the whole adrenal gland and intermingled with the cells containing tyrosine hydroxylase, a marker enzyme for medullary cells. The P45011β-positive and tyrosine hydroxylase-positive cells began to separate on the 20th day, and were completely resolved from each other around the third day after birth. Expression of P450aldo and P45011β, together with that of tyrosine hydroxylase, thus serves as a suitable marker for studying the development of the adrenal gland.
| Original language | English |
|---|---|
| Pages (from-to) | 57-61 |
| Number of pages | 5 |
| Journal | Steroids |
| Volume | 62 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1997 Jan |
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Keywords
- adrenal cortex
- development of adrenal gland
- fetal adrenal
- neonatal adrenal
- P45011β
- P450aldo
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Molecular Biology
Cite this
Expression of cytochromes P450aldo and P45011β in rat adrenal gland during late gestational and neonatal stages. / Mitani, Fumiko; Mukai, Kuniaki; Ogawa, Takayoshi; Miyamoto, Hirokuni; Ishimura, Yuzuru.
In: Steroids, Vol. 62, No. 1, 01.1997, p. 57-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of cytochromes P450aldo and P45011β in rat adrenal gland during late gestational and neonatal stages
AU - Mitani, Fumiko
AU - Mukai, Kuniaki
AU - Ogawa, Takayoshi
AU - Miyamoto, Hirokuni
AU - Ishimura, Yuzuru
PY - 1997/1
Y1 - 1997/1
N2 - The development of the rat adrenal gland during late gestational and neonatal stages was studied by following the expression of aldosterone synthase cytochrome P450 (P450aldo) and glucocorticoid-synthesizing cytochrome P450 (P45011β). Cells expressing P450aldo, a functional marker for the mineralocorticoid-synthesizing zona glomerulosa, were not detected until day 20 of fetal age, i.e., 2 days before birth, although the zona glomerulosa cells were histologically recognizable at the 18th day of gestation. The intensity of P450aldo staining thereafter became stronger with age in the outer portion of the cortex. Cells expressing P45011β, a marker for the glucocorticoid-producing zona fasciculata, were present in the fetal adrenals on the 18th day. P45011β-positive cells were distributed over the whole adrenal gland and intermingled with the cells containing tyrosine hydroxylase, a marker enzyme for medullary cells. The P45011β-positive and tyrosine hydroxylase-positive cells began to separate on the 20th day, and were completely resolved from each other around the third day after birth. Expression of P450aldo and P45011β, together with that of tyrosine hydroxylase, thus serves as a suitable marker for studying the development of the adrenal gland.
AB - The development of the rat adrenal gland during late gestational and neonatal stages was studied by following the expression of aldosterone synthase cytochrome P450 (P450aldo) and glucocorticoid-synthesizing cytochrome P450 (P45011β). Cells expressing P450aldo, a functional marker for the mineralocorticoid-synthesizing zona glomerulosa, were not detected until day 20 of fetal age, i.e., 2 days before birth, although the zona glomerulosa cells were histologically recognizable at the 18th day of gestation. The intensity of P450aldo staining thereafter became stronger with age in the outer portion of the cortex. Cells expressing P45011β, a marker for the glucocorticoid-producing zona fasciculata, were present in the fetal adrenals on the 18th day. P45011β-positive cells were distributed over the whole adrenal gland and intermingled with the cells containing tyrosine hydroxylase, a marker enzyme for medullary cells. The P45011β-positive and tyrosine hydroxylase-positive cells began to separate on the 20th day, and were completely resolved from each other around the third day after birth. Expression of P450aldo and P45011β, together with that of tyrosine hydroxylase, thus serves as a suitable marker for studying the development of the adrenal gland.
KW - adrenal cortex
KW - development of adrenal gland
KW - fetal adrenal
KW - neonatal adrenal
KW - P45011β
KW - P450aldo
UR - http://www.scopus.com/inward/record.url?scp=0031013606&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031013606&partnerID=8YFLogxK
U2 - 10.1016/S0039-128X(96)00160-2
DO - 10.1016/S0039-128X(96)00160-2
M3 - Article
VL - 62
SP - 57
EP - 61
JO - Steroids
JF - Steroids
SN - 0039-128X
IS - 1
ER -