Expression of cytokines and inducible nitric oxide synthese in inflamed gingival tissue

Michiko Hirose, Kazuyuki Ishihara, Atsushi Saito, Taneaki Nakagawa, Satoru Yamada, Katsuji Okuda

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Background: Periodontopathic bacteria induce inflammation of periodontal tissues. The cytokines and nitric oxide released in periodontal lesions have been reported to play a protective role in bacterial infection and to relate to the process of inflammation. To clarify the relationship between colonization of periodontopathic bacteria and cytokines, we evaluated profiles of inflammatory cytokines, chemokine, anti-inflammatory cytokines, and inducible nitric oxide synthase (iNOS) and colonization by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, which are major pathogens of periodontitis. Methods: mRNA expression of cytokines and iNOS in inflamed and healthy gingival tissue was determined using reverse transcription-polymerase chain reaction (RT-PCR), and the relationship between their profiles and the detection of specific bacteria was analyzed. Results: The relative expression of interleukin (IL)-6 and iNOS mRNAs in periodontal lesions was significantly higher than those in healthy individuals. IL-6 mRNA expression was also significantly higher at bleeding on probing (BOP)-positive sites than at BOP-negative sites. The expressions of IL-1α and 1L-8 increased, but IL-10 expression decreased at sites where A. actinomycetemcomitans was detected. We found no correlation between the expression of cytokine and iNOS mRNA and infection by P. gingivalis. Conclusions: The expression of IL-6 may reflect inflammation in gingival tissue, and iNOS may be involved in the inflammatory process in periodontitis. The presence of A. actinomycetemcomitans or P. gingivalis might relate to the different cytokine profiles of IL-1α, IL-8, and IL-10.

Original languageEnglish
Pages (from-to)590-597
Number of pages8
JournalJournal of Periodontology
Volume72
Issue number5
DOIs
Publication statusPublished - 2001 May
Externally publishedYes

Fingerprint

Nitric Oxide
Nitric Oxide Synthase Type II
Cytokines
Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Interleukin-6
Messenger RNA
Periodontitis
Inflammation
Bacteria
Interleukin-1
Interleukin-10
Hemorrhage
Interleukin-8
Chemokines
Bacterial Infections
Reverse Transcription
Anti-Inflammatory Agents
Polymerase Chain Reaction
Infection

Keywords

  • Actinobacillus actinomycetemcomitans
  • Cytokines
  • Nitric oxide synthase
  • Periodontal diseases/microbiology
  • Polymerase chain reaction
  • Porphyromonas gingivalis
  • Reverse transcription

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Expression of cytokines and inducible nitric oxide synthese in inflamed gingival tissue. / Hirose, Michiko; Ishihara, Kazuyuki; Saito, Atsushi; Nakagawa, Taneaki; Yamada, Satoru; Okuda, Katsuji.

In: Journal of Periodontology, Vol. 72, No. 5, 05.2001, p. 590-597.

Research output: Contribution to journalArticle

Hirose, Michiko ; Ishihara, Kazuyuki ; Saito, Atsushi ; Nakagawa, Taneaki ; Yamada, Satoru ; Okuda, Katsuji. / Expression of cytokines and inducible nitric oxide synthese in inflamed gingival tissue. In: Journal of Periodontology. 2001 ; Vol. 72, No. 5. pp. 590-597.
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AU - Yamada, Satoru

AU - Okuda, Katsuji

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