Abstract
Aims: Adamantinomatous craniopharyngioma (ACP) resembles histologically some odontogenic tumours, such as ameloblastoma and calcifying odontogenic cyst. However, there has been no evidence that ACP differentiates also functionally as odontogenic epithelium. The aim of this study was to gain evidence of odontogenic epithelial differentiation in ACP by means of immunohistochemistry. Among normal human tissues, enamel proteins are expressed exclusively in teeth, and lymphoid enhancer factor 1 (LEF1), in co-operation with β-catenin, play an important role in tooth development. The expression of these proteins is therefore indicative of odontogenic epithelial differentiation. Methods and results: The expression of enamel proteins and LEF1 was examined in 10 adamantinomatous and six papillary craniopharyngiomas. All the ACPs showed a variable degree of enamel protein expression, including amelogenin, enamelin and enamelysin, mainly in ghost cells. LEF1 was also heterogeneously expressed in ACPs; remarkably, its expression pattern was identical to that of nuclear β-catenin accumulation. In contrast, none of the papillary craniopharyngiomas expressed enamel proteins or LEF1. Conclusions: These results suggest that ACP consistently shows odontogenic epithelial differentiation. Since ACPs harbour β-catenin mutation, the inappropriate activation of β-catenin/LEF1 complex-dependent transcription may play a critical role in ACP tumorigenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 573-579 |
| Number of pages | 7 |
| Journal | Histopathology |
| Volume | 45 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2004 Dec |
| Externally published | Yes |
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Keywords
- Adamantinomatous craniopharyngioma
- Enamel protein
- LEF1
ASJC Scopus subject areas
- Anatomy
- Pathology and Forensic Medicine
- Cell Biology
Cite this
Expression of enamel proteins and LEF1 in adamantinomatous craniopharyngioma : Evidence for its odontogenic epithelial differentiation. / Sekine, S.; Takata, T.; Shibata, T.; Mori, M.; Morishita, Y.; Noguchi, M.; Uchida, T.; Kanai, Yae; Hirohashi, S.
In: Histopathology, Vol. 45, No. 6, 12.2004, p. 573-579.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of enamel proteins and LEF1 in adamantinomatous craniopharyngioma
T2 - Evidence for its odontogenic epithelial differentiation
AU - Sekine, S.
AU - Takata, T.
AU - Shibata, T.
AU - Mori, M.
AU - Morishita, Y.
AU - Noguchi, M.
AU - Uchida, T.
AU - Kanai, Yae
AU - Hirohashi, S.
PY - 2004/12
Y1 - 2004/12
N2 - Aims: Adamantinomatous craniopharyngioma (ACP) resembles histologically some odontogenic tumours, such as ameloblastoma and calcifying odontogenic cyst. However, there has been no evidence that ACP differentiates also functionally as odontogenic epithelium. The aim of this study was to gain evidence of odontogenic epithelial differentiation in ACP by means of immunohistochemistry. Among normal human tissues, enamel proteins are expressed exclusively in teeth, and lymphoid enhancer factor 1 (LEF1), in co-operation with β-catenin, play an important role in tooth development. The expression of these proteins is therefore indicative of odontogenic epithelial differentiation. Methods and results: The expression of enamel proteins and LEF1 was examined in 10 adamantinomatous and six papillary craniopharyngiomas. All the ACPs showed a variable degree of enamel protein expression, including amelogenin, enamelin and enamelysin, mainly in ghost cells. LEF1 was also heterogeneously expressed in ACPs; remarkably, its expression pattern was identical to that of nuclear β-catenin accumulation. In contrast, none of the papillary craniopharyngiomas expressed enamel proteins or LEF1. Conclusions: These results suggest that ACP consistently shows odontogenic epithelial differentiation. Since ACPs harbour β-catenin mutation, the inappropriate activation of β-catenin/LEF1 complex-dependent transcription may play a critical role in ACP tumorigenesis.
AB - Aims: Adamantinomatous craniopharyngioma (ACP) resembles histologically some odontogenic tumours, such as ameloblastoma and calcifying odontogenic cyst. However, there has been no evidence that ACP differentiates also functionally as odontogenic epithelium. The aim of this study was to gain evidence of odontogenic epithelial differentiation in ACP by means of immunohistochemistry. Among normal human tissues, enamel proteins are expressed exclusively in teeth, and lymphoid enhancer factor 1 (LEF1), in co-operation with β-catenin, play an important role in tooth development. The expression of these proteins is therefore indicative of odontogenic epithelial differentiation. Methods and results: The expression of enamel proteins and LEF1 was examined in 10 adamantinomatous and six papillary craniopharyngiomas. All the ACPs showed a variable degree of enamel protein expression, including amelogenin, enamelin and enamelysin, mainly in ghost cells. LEF1 was also heterogeneously expressed in ACPs; remarkably, its expression pattern was identical to that of nuclear β-catenin accumulation. In contrast, none of the papillary craniopharyngiomas expressed enamel proteins or LEF1. Conclusions: These results suggest that ACP consistently shows odontogenic epithelial differentiation. Since ACPs harbour β-catenin mutation, the inappropriate activation of β-catenin/LEF1 complex-dependent transcription may play a critical role in ACP tumorigenesis.
KW - Adamantinomatous craniopharyngioma
KW - Enamel protein
KW - LEF1
UR - http://www.scopus.com/inward/record.url?scp=11144350252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11144350252&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2559.2004.02029.x
DO - 10.1111/j.1365-2559.2004.02029.x
M3 - Article
C2 - 15569047
AN - SCOPUS:11144350252
VL - 45
SP - 573
EP - 579
JO - Histopathology
JF - Histopathology
SN - 0309-0167
IS - 6
ER -