Expression of Ets-1 in human clear cell renal cell carcinomas: Implications for angiogenesis

Shuji Mikami, Mototsugu Oya, Ryuichi Mizuno, Masaru Murai, Makio Mukai, Yasunori Okada

Research output: Contribution to journalArticle

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Abstract

Expression of vascular endothelial growth factor (VEGF) has been reported in renal cell carcinoma (RCC), a highly angiogenic carcinoma. However, little or no information is available on the expression of Ets-1, which is one of the target molecules of VEGF. In the present study, we examined the expression of Ets-1 and VEGF in RCC by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), and correlations between expression and the microvessel density (MVD) were evaluated. Ets-1 was immunolocalized to carcinoma cells and endothelial cells of the microvessels in clear cell RCC, but not in papillary RCC. Immunohistochemical Ets-1 expression and MVD were significantly higher in clear cell RCC than in papillary RCC. Predominant mRNA expression of Ets-1 in clear cell RCC was confirmed by RT-PCR. The expression of Ets-1 correlated directly with MVD in clear cell RCC. Hypoxic treatment upregulated the mRNA expression of Ets-1 and VEGF in cell lines derived from clear cell RCC, suggesting that hypoxia is a key regulator for these molecules. These results demonstrate the expression of Ets-1 in human clear cell RCC and suggest the possibility that Ets-1 is involved in angiogenesis in clear cell RCC.

Original languageEnglish
Pages (from-to)875-882
Number of pages8
JournalCancer Science
Volume97
Issue number9
DOIs
Publication statusPublished - 2006 Sep

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Renal Cell Carcinoma
Microvessels
Vascular Endothelial Growth Factor A
Reverse Transcription
Carcinoma
Polymerase Chain Reaction
Messenger RNA
Endothelial Cells
Immunohistochemistry
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Expression of Ets-1 in human clear cell renal cell carcinomas : Implications for angiogenesis. / Mikami, Shuji; Oya, Mototsugu; Mizuno, Ryuichi; Murai, Masaru; Mukai, Makio; Okada, Yasunori.

In: Cancer Science, Vol. 97, No. 9, 09.2006, p. 875-882.

Research output: Contribution to journalArticle

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