Expression of G1→S transition regulatory molecules in human urothelial cancer

Mototsugu Oya, Bettina Schmidt, Bernd J. Schmitz-Dräger, Wolfgang A. Schulz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Growth of cancer cells is characterized by accelerated passage through the cell cycle, which is often caused by deregulation of the G1→S transition. In this study the expression of G1→S transition regulatory molecules was analyzed in 32 transitional cell carcinoma specimens and fifteen normal tissues obtained by cystectomy or nephroureterectomy of mainly locally advanced tumors, as well as six bladder cancer cell lines. Expression of mRNAs for cyclins D1 and D2 and cyclin-dependent kinases (CDK) 2 and 4 was investigated by quantitative reverse transcription-polymerase chain reaction. Overexpression of cyclin D1 compared to normal mucosa was observed in 3 tumors (9.4%), but in neither of the cell lines. All tumors with overexpression were moderately differentiated (G2) pT1 or pT2 tumors, and thus among the less advanced specimens. Cyclin D2 was not expressed in normal bladder mucosa or in tumors. The expression of CDK4 mRNA varied within the same range in mucosa, tumors, and cell lines, CDK2 mRNA expression varied more strongly and was diminished in individual tumors and in four cell lines. It is concluded that cyclin D1 overexpression can play an important role in the early stage of urothelial tumorigenesis, driving cell proliferation. Ectopic expression of cyclin D2 or amplification of CDK4 does not occur at a significant frequency in urothelial carcinomas. Different expression patterns of cyclin D1 and CDK2 indicate heterogeneity in the mechanisms of G1→S transition deregulation in individual bladder tumors which may elicit differences in their biological and clinical behavior.

Original languageEnglish
Pages (from-to)719-726
Number of pages8
JournalJapanese Journal of Cancer Research
Volume89
Issue number7
Publication statusPublished - 1998 Jul
Externally publishedYes

Fingerprint

Cyclin D1
Cyclin D2
Neoplasms
Mucous Membrane
Urinary Bladder Neoplasms
Cell Line
Messenger RNA
Cyclin-Dependent Kinase 4
Cyclin-Dependent Kinase 2
Transitional Cell Carcinoma
Cystectomy
Tumor Cell Line
Reverse Transcription
Cell Cycle
Carcinogenesis
Urinary Bladder
Cell Proliferation
Carcinoma
Polymerase Chain Reaction
Growth

Keywords

  • CDK2
  • CDK4
  • Cyclin D
  • Rb
  • RT-PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Oya, M., Schmidt, B., Schmitz-Dräger, B. J., & Schulz, W. A. (1998). Expression of G1→S transition regulatory molecules in human urothelial cancer. Japanese Journal of Cancer Research, 89(7), 719-726.

Expression of G1→S transition regulatory molecules in human urothelial cancer. / Oya, Mototsugu; Schmidt, Bettina; Schmitz-Dräger, Bernd J.; Schulz, Wolfgang A.

In: Japanese Journal of Cancer Research, Vol. 89, No. 7, 07.1998, p. 719-726.

Research output: Contribution to journalArticle

Oya, M, Schmidt, B, Schmitz-Dräger, BJ & Schulz, WA 1998, 'Expression of G1→S transition regulatory molecules in human urothelial cancer', Japanese Journal of Cancer Research, vol. 89, no. 7, pp. 719-726.
Oya, Mototsugu ; Schmidt, Bettina ; Schmitz-Dräger, Bernd J. ; Schulz, Wolfgang A. / Expression of G1→S transition regulatory molecules in human urothelial cancer. In: Japanese Journal of Cancer Research. 1998 ; Vol. 89, No. 7. pp. 719-726.
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