Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver

S. Moriya, S. Nagata, Hirokazu Yokoyama, Shinzo Kato, Y. Horie, T. Ito, Y. Ebihara, H. Ishii

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Gamma-glutamyl transpeptidase (γ-GT) is the enzyme in the γ-glutamyl cycle which mediates the first step of glutathione degradation. Recently, it has been reported that diethyl maleate, a glutathione-depleting agent, enhanced hepatic γ-GT activity. In this study, we examined how γ-GT mRNA was expressed after the depletion of glutathione by using the RT-PCR method and in situ hybridization technique with digoxigenin-labeled oligonucleotide probe. We could detect hepatic γ-GT mRNA in the epithelial cell of bile duct and the cytoplasm of hepatocytes in the periportal area, even in control rats. Furthermore, we found that the expression of γ-GT mRNA was expanded to hepatocytes of a whole lobule 12 hr after depletion of glutathione by diethyl maleate, followed by the return to the pretreatment condition under which γ-GT mRNA appears to be controlled in correlation with hepatic glutathione levels. These results suggest that the depletion of glutathione may induce hepatic γ-GT activity through an increased synthesis of its mRNA.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalAlcohol and Alcoholism
Volume29
Issue numberSUPPL. 1
Publication statusPublished - 1994

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gamma-Glutamyltransferase
Liver
Glutathione
Rats
diethyl maleate
Messenger RNA
Hepatocytes
Rat control
Digoxigenin
Oligonucleotide Probes
Bile Ducts
Ducts
In Situ Hybridization
Cytoplasm
Epithelial Cells
Degradation
Polymerase Chain Reaction
Enzymes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Moriya, S., Nagata, S., Yokoyama, H., Kato, S., Horie, Y., Ito, T., ... Ishii, H. (1994). Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver. Alcohol and Alcoholism, 29(SUPPL. 1), 107-111.

Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver. / Moriya, S.; Nagata, S.; Yokoyama, Hirokazu; Kato, Shinzo; Horie, Y.; Ito, T.; Ebihara, Y.; Ishii, H.

In: Alcohol and Alcoholism, Vol. 29, No. SUPPL. 1, 1994, p. 107-111.

Research output: Contribution to journalArticle

Moriya, S, Nagata, S, Yokoyama, H, Kato, S, Horie, Y, Ito, T, Ebihara, Y & Ishii, H 1994, 'Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver', Alcohol and Alcoholism, vol. 29, no. SUPPL. 1, pp. 107-111.
Moriya, S. ; Nagata, S. ; Yokoyama, Hirokazu ; Kato, Shinzo ; Horie, Y. ; Ito, T. ; Ebihara, Y. ; Ishii, H. / Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver. In: Alcohol and Alcoholism. 1994 ; Vol. 29, No. SUPPL. 1. pp. 107-111.
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