Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver

S. Moriya, S. Nagata, H. Yokoyama, S. Kato, Y. Horie, T. Ito, Y. Ebihara, H. Ishii

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Abstract

Gamma-glutamyl transpeptidase (γ-GT) is the enzyme in the γ-glutamyl cycle which mediates the first step of glutathione degradation. Recently, it has been reported that diethyl maleate, a glutathione-depleting agent, enhanced hepatic γ-GT activity. In this study, we examined how γ-GT mRNA was expressed after the depletion of glutathione by using the RT-PCR method and in situ hybridization technique with digoxigenin-labeled oligonucleotide probe. We could detect hepatic γ-GT mRNA in the epithelial cell of bile duct and the cytoplasm of hepatocytes in the periportal area, even in control rats. Furthermore, we found that the expression of γ-GT mRNA was expanded to hepatocytes of a whole lobule 12 hr after depletion of glutathione by diethyl maleate, followed by the return to the pretreatment condition under which γ-GT mRNA appears to be controlled in correlation with hepatic glutathione levels. These results suggest that the depletion of glutathione may induce hepatic γ-GT activity through an increased synthesis of its mRNA.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalAlcohol and Alcoholism
Volume29
Issue numberSUPPL. 1
Publication statusPublished - 1994 Dec 1

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

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    Moriya, S., Nagata, S., Yokoyama, H., Kato, S., Horie, Y., Ito, T., Ebihara, Y., & Ishii, H. (1994). Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver. Alcohol and Alcoholism, 29(SUPPL. 1), 107-111.