Expression of hepatitis B surface antigen in Chang cells transfected with hepatitis B virus DNA.

Hidetsugu Saito, T. Morizane, T. Watanabe, T. Kagawa, S. Matsumoto, N. Iwabuchi, N. Kumagai, K. Tsuchimoto, M. Tsuchiya

Research output: Contribution to journalArticle

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Abstract

To investigate hepatitis B virus (HBV) biology in vitro, the transfection of recirculized HBV DNA into Chang cell line was performed. Linear HBV DNA was isolated from recombinant HBV DNA, pHBR105, which includes the whole genome of HBV and was recirculized. Chang cells were transfected with this recirculized HBV DNA by the two different procedures of calcium/phosphate coprecipitation and electroporation. After the transfection, the presence of large nucleated cells with multinuclei and ground-glass cytoplasma were noticed and these cells seemed to proliferate faster than untreated Chang cells. Transient expression of hepatitis B virus surface antigen (HBsAg) was demonstrated in cytoplasma of transfected cells by indirect immunofluorescence. HBsAg was not detected in the culture supernatants by radioimmunoassay. The extra-chromosomal HBV DNA was detected in the transfected cells by both procedures 7 weeks after the transfection by Southern blot analysis but it was lost 4 weeks after that. It was demonstrated that it was possible to transfect Chang cells with HBV DNA and that DNA was functioning to express HBsAg transiently.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalKeio Journal of Medicine
Volume39
Issue number2
Publication statusPublished - 1990 Jun

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Hepatitis B Surface Antigens
Hepatitis B virus
DNA
Transfection
Electroporation
Indirect Fluorescent Antibody Technique
Southern Blotting
Radioimmunoassay
Glass
Genome
Cell Line

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Saito, H., Morizane, T., Watanabe, T., Kagawa, T., Matsumoto, S., Iwabuchi, N., ... Tsuchiya, M. (1990). Expression of hepatitis B surface antigen in Chang cells transfected with hepatitis B virus DNA. Keio Journal of Medicine, 39(2), 79-85.

Expression of hepatitis B surface antigen in Chang cells transfected with hepatitis B virus DNA. / Saito, Hidetsugu; Morizane, T.; Watanabe, T.; Kagawa, T.; Matsumoto, S.; Iwabuchi, N.; Kumagai, N.; Tsuchimoto, K.; Tsuchiya, M.

In: Keio Journal of Medicine, Vol. 39, No. 2, 06.1990, p. 79-85.

Research output: Contribution to journalArticle

Saito, H, Morizane, T, Watanabe, T, Kagawa, T, Matsumoto, S, Iwabuchi, N, Kumagai, N, Tsuchimoto, K & Tsuchiya, M 1990, 'Expression of hepatitis B surface antigen in Chang cells transfected with hepatitis B virus DNA.', Keio Journal of Medicine, vol. 39, no. 2, pp. 79-85.
Saito H, Morizane T, Watanabe T, Kagawa T, Matsumoto S, Iwabuchi N et al. Expression of hepatitis B surface antigen in Chang cells transfected with hepatitis B virus DNA. Keio Journal of Medicine. 1990 Jun;39(2):79-85.
Saito, Hidetsugu ; Morizane, T. ; Watanabe, T. ; Kagawa, T. ; Matsumoto, S. ; Iwabuchi, N. ; Kumagai, N. ; Tsuchimoto, K. ; Tsuchiya, M. / Expression of hepatitis B surface antigen in Chang cells transfected with hepatitis B virus DNA. In: Keio Journal of Medicine. 1990 ; Vol. 39, No. 2. pp. 79-85.
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