Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines

Jun Ma, Kaneyuki Kubushiro, Yasuhiro Tashima, Katsumi Tsukazaki, Yasuhiro Udagawa, Shiro Nozawa, Michiko N. Fukuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We examined the expression of β 1,4-GT gene products in 11 gynecological cancer cell lines. A 4.7 kb mRNA and protein (54,000 Da and 57,000 Da) were detected by Northern blot and Western blot. Immunocytochemical staining revealed that β 1,4-GT was localized in the Golgi or ER of tumor cells. An intense β 1,4-GT mRNA signal was detected in ovarian and cervical cancer cells, whereas the level of β 1,4-GT mRNA was very low in uterine endometrial cancer cells. We also confirmed that expression of β 1,4-GT mRNA corresponded to expression of β 1,4-GT protein. These results suggest that expression of the β 1,4-GT gene products is higher in human cervical and ovarian cancer cells than in uterine endometrial cancer cells.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalInternational journal of oncology
Volume11
Issue number1
Publication statusPublished - 1997

Keywords

  • Gynecological cancer
  • Human β 1,4-galactosyltransferase
  • Immunocytochemical staining
  • Northern blot analysis
  • Western blot analysis
  • cDNA probes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Ma, J., Kubushiro, K., Tashima, Y., Tsukazaki, K., Udagawa, Y., Nozawa, S., & Fukuda, M. N. (1997). Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines. International journal of oncology, 11(1), 117-122.