Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines

Jun Ma, Kaneyuki Kubushiro, Yasuhiro Tashima, Katsumi Tsukazaki, Yasuhiro Udagawa, Shiro Nozawa, Michiko N. Fukuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We examined the expression of β 1,4-GT gene products in 11 gynecological cancer cell lines. A 4.7 kb mRNA and protein (54,000 Da and 57,000 Da) were detected by Northern blot and Western blot. Immunocytochemical staining revealed that β 1,4-GT was localized in the Golgi or ER of tumor cells. An intense β 1,4-GT mRNA signal was detected in ovarian and cervical cancer cells, whereas the level of β 1,4-GT mRNA was very low in uterine endometrial cancer cells. We also confirmed that expression of β 1,4-GT mRNA corresponded to expression of β 1,4-GT protein. These results suggest that expression of the β 1,4-GT gene products is higher in human cervical and ovarian cancer cells than in uterine endometrial cancer cells.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalInternational Journal of Oncology
Volume11
Issue number1
Publication statusPublished - 1997

Fingerprint

Galactosyltransferases
Cell Line
Messenger RNA
Uterine Neoplasms
Neoplasms
Endometrial Neoplasms
Uterine Cervical Neoplasms
Ovarian Neoplasms
Northern Blotting
Genes
Proteins
Western Blotting
Staining and Labeling

Keywords

  • cDNA probes
  • Gynecological cancer
  • Human β 1,4-galactosyltransferase
  • Immunocytochemical staining
  • Northern blot analysis
  • Western blot analysis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ma, J., Kubushiro, K., Tashima, Y., Tsukazaki, K., Udagawa, Y., Nozawa, S., & Fukuda, M. N. (1997). Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines. International Journal of Oncology, 11(1), 117-122.

Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines. / Ma, Jun; Kubushiro, Kaneyuki; Tashima, Yasuhiro; Tsukazaki, Katsumi; Udagawa, Yasuhiro; Nozawa, Shiro; Fukuda, Michiko N.

In: International Journal of Oncology, Vol. 11, No. 1, 1997, p. 117-122.

Research output: Contribution to journalArticle

Ma, J, Kubushiro, K, Tashima, Y, Tsukazaki, K, Udagawa, Y, Nozawa, S & Fukuda, MN 1997, 'Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines', International Journal of Oncology, vol. 11, no. 1, pp. 117-122.
Ma J, Kubushiro K, Tashima Y, Tsukazaki K, Udagawa Y, Nozawa S et al. Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines. International Journal of Oncology. 1997;11(1):117-122.
Ma, Jun ; Kubushiro, Kaneyuki ; Tashima, Yasuhiro ; Tsukazaki, Katsumi ; Udagawa, Yasuhiro ; Nozawa, Shiro ; Fukuda, Michiko N. / Expression of human β 1,4-galactosyltransferase in gynecological cancer cell lines. In: International Journal of Oncology. 1997 ; Vol. 11, No. 1. pp. 117-122.
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