Expression of immunoreactivity to neurokinin-1 receptor by subsets of cranial parasympathetic neurons

Correlation with neuropeptides, nitric oxide synthase, and pathways

Toshihiko Shimizu, J. L. Morris, I. L. Gibbins

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We examined the patterns of coexistence of immunoreactivity to the neurokinin-1 (NK1) tachykinin receptor, nitric oxide synthase, and neuropeptides in the sphenopalatine and otic ganglia of guinea pigs using a combination of multiple-labeling immunohistochemistry and pathway tracing in vitro. Most neurons had immunoreactivity to vasoactive intestinal peptide (85-96%) and neuropeptide Y (60%). Subpopulations of vasoactive intestinal peptide-immunoreactive neurons also had immunoreactivity to nitric oxide synthase (37-48%) or enkephalin (25-35%), but these formed mutually exclusive populations. Almost all neurons expressing NK1 receptor immunoreactivity contained immunoreactivity to enkephalin, vasoactive intestinal peptide, and neuropeptide Y, but not nitric oxide synthase. Using a combination of retrograde axonal tracing and axonal crushing, we found that most neurons with immunoreactivity to nitric oxide synthase projected along the nasopalatine and moidal nerves to the nasal mucosa. In contrast, most neurons with immunoreactivity to enkephalin followed the zygomatic nerve to the facial skin and lacrimal gland. Based on their peptide content, we conclude that the neurons with immunoreactivity to enkephalin and NK1 receptor projected selectively to the skin. In both the sphenopalatine and the otic ganglia, about half of the neurons with NK1 receptor immunoreactivity were surrounded by varicose nerve fibers with substance P immunoreactivity. Many of these fibers are likely to have originated in the trigeminal ganglion. Taken together, these observations establish a strong anatomical basis for a range of interactions between trigeminal and cranial parasympathetic pathways that may underlie pathophysiological conditions such as trigeminal neuralgia.

Original languageEnglish
Pages (from-to)293-306
Number of pages14
JournalExperimental Neurology
Volume172
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Neurokinin-1 Receptors
Neuropeptides
Nitric Oxide Synthase
Enkephalins
Neurons
Vasoactive Intestinal Peptide
Parasympathetic Ganglia
Neuropeptide Y
Tachykinins
Trigeminal Ganglion
Lacrimal Apparatus
Trigeminal Neuralgia
Skin
Nasal Mucosa
Facial Nerve
Substance P
Nerve Fibers
Guinea Pigs
Immunohistochemistry
Peptides

Keywords

  • Enkephalin
  • Neurokinin-1 receptor
  • Nitric oxide synthase
  • Otic ganglion
  • Parasympathetic
  • Sphenopalatine (pterygopalatine)ganglion
  • Substance P
  • Trigeminal neuralgia

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Expression of immunoreactivity to neurokinin-1 receptor by subsets of cranial parasympathetic neurons : Correlation with neuropeptides, nitric oxide synthase, and pathways. / Shimizu, Toshihiko; Morris, J. L.; Gibbins, I. L.

In: Experimental Neurology, Vol. 172, No. 2, 2001, p. 293-306.

Research output: Contribution to journalArticle

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