Expression of interleukin-6 is suppressed by inhibition of voltage-sensitive Na+/Ca2+ channels after cerebral ischemia

Shigeaki Suzuki, Kortaro Tanaka, Shigeru Nogawa, Tomohisa Dembo, Arifumi Kosakai, Yasuo Fukuuchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Expression of interleukin-6 (IL-6), a neurotrophic cytokine, is up-regulated after cerebral ischemia, but the underlying mechanism of the up-regulation remains unclear. NS-7 is a novel blocker of voltage-sensitive Ca2+ and Na+ channels and is known to reduce cerebral damage by ischemia. The present study was undertaken to examine the association between increases in intracellular Ca2+ concentration induced by membrane depolarization and IL-6 induction. IL-6 expression in rat brain was investigated by immunohistochemistry and Western blot analysis following 3.5-48 h of reperfusion after 1.5 h of occlusion of the middle cerebral artery. NS-7 (1 mg/kg; NS-7 group) or saline (saline group) was injected i.v. 5 min after the start of reperfusion. The saline group showed clear IL-6 expression in various cortical regions, which peaked at 24h of reperfusion. By contrast, IL-6 expression was significantly suppressed in the NS-7 group throughout the reperfusion period. Microglia activation was also reduced in the NS-7 group. These findings suggest that IL-6 expression may be up-regulated by the increased intracellular Ca2+ concentration triggered by membrane depolarization after cerebral ischemia. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)2565-2569
Number of pages5
JournalNeuroReport
Volume11
Issue number11
Publication statusPublished - 2000 Aug 3

Fingerprint

Brain Ischemia
Interleukin-6
Reperfusion
Membranes
Middle Cerebral Artery Infarction
Microglia
Up-Regulation
Western Blotting
Immunohistochemistry
4-(4-fluorophenyl)-2-methyl-6-(5-piperidinopentyloxy)pyrimidine hydrochloride
Cytokines
Brain

Keywords

  • Ca channel
  • Cerebral ischemia
  • Immunohistochemistry
  • Interleukin-6
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Suzuki, S., Tanaka, K., Nogawa, S., Dembo, T., Kosakai, A., & Fukuuchi, Y. (2000). Expression of interleukin-6 is suppressed by inhibition of voltage-sensitive Na+/Ca2+ channels after cerebral ischemia. NeuroReport, 11(11), 2565-2569.

Expression of interleukin-6 is suppressed by inhibition of voltage-sensitive Na+/Ca2+ channels after cerebral ischemia. / Suzuki, Shigeaki; Tanaka, Kortaro; Nogawa, Shigeru; Dembo, Tomohisa; Kosakai, Arifumi; Fukuuchi, Yasuo.

In: NeuroReport, Vol. 11, No. 11, 03.08.2000, p. 2565-2569.

Research output: Contribution to journalArticle

Suzuki, S, Tanaka, K, Nogawa, S, Dembo, T, Kosakai, A & Fukuuchi, Y 2000, 'Expression of interleukin-6 is suppressed by inhibition of voltage-sensitive Na+/Ca2+ channels after cerebral ischemia', NeuroReport, vol. 11, no. 11, pp. 2565-2569.
Suzuki, Shigeaki ; Tanaka, Kortaro ; Nogawa, Shigeru ; Dembo, Tomohisa ; Kosakai, Arifumi ; Fukuuchi, Yasuo. / Expression of interleukin-6 is suppressed by inhibition of voltage-sensitive Na+/Ca2+ channels after cerebral ischemia. In: NeuroReport. 2000 ; Vol. 11, No. 11. pp. 2565-2569.
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