Expression of interleukin-6 is suppressed by inhibition of voltage-sensitive Na+/Ca2+ channels after cerebral ischemia

Shigeaki Suzuki, Kortaro Tanaka, Shigeru Nogawa, Tomohisa Dembo, Arifumi Kosakai, Yasuo Fukuuchi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Expression of interleukin-6 (IL-6), a neurotrophic cytokine, is up-regulated after cerebral ischemia, but the underlying mechanism of the up-regulation remains unclear. NS-7 is a novel blocker of voltage-sensitive Ca2+ and Na+ channels and is known to reduce cerebral damage by ischemia. The present study was undertaken to examine the association between increases in intracellular Ca2+ concentration induced by membrane depolarization and IL-6 induction. IL-6 expression in rat brain was investigated by immunohistochemistry and Western blot analysis following 3.5-48 h of reperfusion after 1.5 h of occlusion of the middle cerebral artery. NS-7 (1 mg/kg; NS-7 group) or saline (saline group) was injected i.v. 5 min after the start of reperfusion. The saline group showed clear IL-6 expression in various cortical regions, which peaked at 24h of reperfusion. By contrast, IL-6 expression was significantly suppressed in the NS-7 group throughout the reperfusion period. Microglia activation was also reduced in the NS-7 group. These findings suggest that IL-6 expression may be up-regulated by the increased intracellular Ca2+ concentration triggered by membrane depolarization after cerebral ischemia. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)2565-2569
Number of pages5
Issue number11
Publication statusPublished - 2000 Aug 3


  • Ca channel
  • Cerebral ischemia
  • Immunohistochemistry
  • Interleukin-6
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)


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