Abstract
In situ hybridization (ISH) of the expression of matrix metalloproteinase-1 (MMP-1) in areas of local invasion of head and neck tumours was performed. Expression of MMP-1 mRNA was found in stromal inflammatory cells adjacent to the tumour nests, apparently in eosinophils. MMP-1 mRNA expression was seen only in cases with moderate or greater stromal eosinophilia. MMP-1 mRNA was detected in benign papilloma and verrucous carcinoma. In squamous cell carcinomas, expression of the gene was detected only in well-differentiated cases. Expression of MMP-1 mRNA was not related to the grade of malignancy, and appeared in stromal eosinophils, suggesting to us that it is involved in a remodelling process in the reaction of the host to tumour invasion. ISH examination of the expression of interleukin-5 (IL-5), the activating factor for eosinophils, was also performed. In the tumour, its expression was consistent with the location of eosinophils, as with MMP-1 mRNA, suggesting an autocrine mechanism.
| Original language | English |
|---|---|
| Pages (from-to) | 305-310 |
| Number of pages | 6 |
| Journal | Virchows Archiv |
| Volume | 431 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1997 |
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Keywords
- Eosinophil
- Head and neck tumour
- IL-5
- In situ hybridization
- Matrix metalloproteinase
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Expression of matrix metalloproteinase-1 mRNA related to eosinophilia and interleukin-5 gene expression in head and neck tumour tissue. / Ono, Yoshihiro; Fujii, Masato; Kameyama, Kaori; Otani, Yoshihide; Sakurai, Yoshihiko; Kanzaki, Jin.
In: Virchows Archiv, Vol. 431, No. 5, 1997, p. 305-310.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of matrix metalloproteinase-1 mRNA related to eosinophilia and interleukin-5 gene expression in head and neck tumour tissue
AU - Ono, Yoshihiro
AU - Fujii, Masato
AU - Kameyama, Kaori
AU - Otani, Yoshihide
AU - Sakurai, Yoshihiko
AU - Kanzaki, Jin
PY - 1997
Y1 - 1997
N2 - In situ hybridization (ISH) of the expression of matrix metalloproteinase-1 (MMP-1) in areas of local invasion of head and neck tumours was performed. Expression of MMP-1 mRNA was found in stromal inflammatory cells adjacent to the tumour nests, apparently in eosinophils. MMP-1 mRNA expression was seen only in cases with moderate or greater stromal eosinophilia. MMP-1 mRNA was detected in benign papilloma and verrucous carcinoma. In squamous cell carcinomas, expression of the gene was detected only in well-differentiated cases. Expression of MMP-1 mRNA was not related to the grade of malignancy, and appeared in stromal eosinophils, suggesting to us that it is involved in a remodelling process in the reaction of the host to tumour invasion. ISH examination of the expression of interleukin-5 (IL-5), the activating factor for eosinophils, was also performed. In the tumour, its expression was consistent with the location of eosinophils, as with MMP-1 mRNA, suggesting an autocrine mechanism.
AB - In situ hybridization (ISH) of the expression of matrix metalloproteinase-1 (MMP-1) in areas of local invasion of head and neck tumours was performed. Expression of MMP-1 mRNA was found in stromal inflammatory cells adjacent to the tumour nests, apparently in eosinophils. MMP-1 mRNA expression was seen only in cases with moderate or greater stromal eosinophilia. MMP-1 mRNA was detected in benign papilloma and verrucous carcinoma. In squamous cell carcinomas, expression of the gene was detected only in well-differentiated cases. Expression of MMP-1 mRNA was not related to the grade of malignancy, and appeared in stromal eosinophils, suggesting to us that it is involved in a remodelling process in the reaction of the host to tumour invasion. ISH examination of the expression of interleukin-5 (IL-5), the activating factor for eosinophils, was also performed. In the tumour, its expression was consistent with the location of eosinophils, as with MMP-1 mRNA, suggesting an autocrine mechanism.
KW - Eosinophil
KW - Head and neck tumour
KW - IL-5
KW - In situ hybridization
KW - Matrix metalloproteinase
UR - http://www.scopus.com/inward/record.url?scp=0030708788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030708788&partnerID=8YFLogxK
U2 - 10.1007/s004280050103
DO - 10.1007/s004280050103
M3 - Article
C2 - 9463570
AN - SCOPUS:0030708788
VL - 431
SP - 305
EP - 310
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 5
ER -