Expression of matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) induced by tumour necrosis factor α correlates with metastatic ability in a human osteosarcoma cell line

A. Kawashima, I. Nakanishi, Y. Okada, H. Tsuchiya, A. Roessner, Ken'ichi Obata

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We have examined the correlation between matrix metalloproteinase (MMP) expression and metastatic properties of a low metastatic osteosarcoma cell line, osteosarcoma takase (OST), under stimulation by tumour necrosis factor α (TNFα). In vivo, OST cells exhibited significantly increased colonization in the lungs of nude mice in a dose-dependent manner when they were treated by TNFα prior to injection. In vitro, TNFα enhanced tumour cell invasion through the reconstituted basement membrane in a transwell chamber up to 2.5-fold. Gelatin zymography and sandwich enzyme immunoassays demonstrated marked production of MMP-9 [92-kDa gelatinase/type IV collagenase (gelatinase B)] but not MMP-2 [72-kDa gelatinase/type IV collagenase (gelatinase A)], MMP-3 (stromelysin-1) or MMP-7 (matrilysin). Motility of the tumour cells and adhesion to cultured endothelial cells were slightly increased by the TNFα treatment up to 1.6-fold and 1.4-fold, respectively, while the growth rate was decreased. These results suggest that upregulation of MMP-9 together with enhanced motility and endothelial adhesion contribute to the increased metastatic ability of OST cells induced by TNFα treatment.

Original languageEnglish
Pages (from-to)547-552
Number of pages6
JournalVirchows Archiv
Volume424
Issue number5
DOIs
Publication statusPublished - 1994 Jun
Externally publishedYes

Keywords

  • Invasion
  • Matrix metalloproteinase
  • Metastasis
  • Osteosarcoma
  • Tumour necrosis factor α

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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