Expression of membrane-type matrix metalloproteinase in human gastric carcinomas

H. Nomura, H. Sato, M. Seiki, M. Mat, Y. Okada

Research output: Contribution to journalArticle

295 Citations (Scopus)

Abstract

We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.

Original languageEnglish
Pages (from-to)3263-3266
Number of pages4
JournalCancer Research
Volume55
Issue number15
Publication statusPublished - 1995

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Membrane-Associated Matrix Metalloproteinases
Stomach
Carcinoma
Matrix Metalloproteinases
Microdissection
Enzyme Precursors
Gelatin
Gastric Mucosa
Northern Blotting
Culture Media
progelatinase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nomura, H., Sato, H., Seiki, M., Mat, M., & Okada, Y. (1995). Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. Cancer Research, 55(15), 3263-3266.

Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. / Nomura, H.; Sato, H.; Seiki, M.; Mat, M.; Okada, Y.

In: Cancer Research, Vol. 55, No. 15, 1995, p. 3263-3266.

Research output: Contribution to journalArticle

Nomura, H, Sato, H, Seiki, M, Mat, M & Okada, Y 1995, 'Expression of membrane-type matrix metalloproteinase in human gastric carcinomas', Cancer Research, vol. 55, no. 15, pp. 3263-3266.
Nomura H, Sato H, Seiki M, Mat M, Okada Y. Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. Cancer Research. 1995;55(15):3263-3266.
Nomura, H. ; Sato, H. ; Seiki, M. ; Mat, M. ; Okada, Y. / Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. In: Cancer Research. 1995 ; Vol. 55, No. 15. pp. 3263-3266.
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